摘要
目的观察补阳还五汤精简方对大鼠脑缺血后血管新生及核转录因子E2相关因子2(Nrf2)/血红素加氧酶-1(HO-1)信号途径的影响。方法 120只SD大鼠随机分成假手术组、模型组、补阳还五汤组、补阳还五汤精简方组。采用大脑中动脉线栓法建立局灶性脑缺血模型,各组给予相应处理。在d 1、3、7三个时间点取脑组织进行检测,免疫组织化学法检测血浆Ⅷ因子相关抗原(v WF),测定微血管密度(MVD)。采用Real-time PCR和Western blot法检测大鼠脑组织中Nrf2、HO-1基因及蛋白表达。结果 1与假手术组比,模型组v WF阳性表达微血管在d 3时表达明显升高(P<0.05),给药组d 7与模型组比较差异有显著性(P<0.05);2假手术组Nrf2、HO-1mRNA及蛋白呈少量表达,模型组Nrf2蛋白表达水平d 3与同时间点假手术组比较差异有统计学意义(P<0.01),各给药组能上调Nrf2mRNA及蛋白表达,其中Nrf2mRNA在d 7,Nrf2蛋白表达在d 1及d 7上调最明显,与同时间点模型组比较差异具有统计学意义(P<0.01)。模型组HO-1mRNA及蛋白在d 7与同时间点假手术组比较差异有统计学意义(P<0.05),各给药组HO-1mRNA在d 3,HO-1蛋白在d 3及d 7上调最明显,与同时段模型组比较差异具有统计学意义(P<0.05,P<0.01)。结论补阳还五汤精简方促进脑缺血后血管新生,这一作用可能与调控Nrf2/HO-1信号途径的表达有关。
Aim To observe the effects of thin recipe of Buyang Huanwu Decoction on angiogenesis and the signal pathway of Nrf2 / HO-1 after cerebral ischemic injury in rats. Methods Totally 120 SD rats were randomly divided into four groups: sham operationgroup,model group,Buyang Huanwu Decoction group and thin recipe of Buyang Huanwu Decoction group.The focal cerebral ischemia rat model was established by middle cerebral arterial occlusion. Each group was treated with corresponding treatment. Each group wasdetected after cerebral ischemia for day 1,day 3 and day 7,respectively. Immunohistochemical method was used to detect the plasma levels of factor VIII related antigen( v WF),determination of microvessel density( MVD). The expression of Nrf2,HO-1 gene and protein in brain tissues was detected by Real-Time PCR( RT-PCR) and Western blot. Results 1 Compared with sham-operation group,the expression of v WF in the model group was significantly increased on day 3( P< 0. 05). Compared with model group,the expression levels increased differently in each drug group on day 7( P < 0. 05). 2 The expression of Nrf2,HO-1 gene and protein in sham operation group showed a small amount of gamma expression. Compared with sham operation group at the same time point,the expression of Nrf2 protein was significantly increased on day 3( P <0. 01). Compared with model group at the same time point,the Nrf2 mRNA and protein expression was up-regulated in each drug group. The Nrf2 mRNA on day1,the Nrf2 protein on day 1 and day 7 were significantly increased( P < 0. 01). Compared with sham operation group at the same time point,the expression of HO-1mRNA and protein in the model group was significantly increased on day 7( P < 0. 05). Compared with model group at the same time point,the HO-1mRNA on day 3,the HO-1 protein on day 3 and day 7 in each drug group were significantly increased( P < 0. 05,P <0. 01). Conclusions The thin recipe of Buyang Huanwu Decoction promotes brain angiogenesis after ischemia. The effect may be related wih the expression
出处
《中国药理学通报》
CAS
CSCD
北大核心
2016年第1期123-128,共6页
Chinese Pharmacological Bulletin
基金
国家重点基础研究发展计划(973计划)项目(No 2012CB723503)
湖南省科学技术厅科技计划一般项目(No2013SK3107)
湖南省中医内科学省部共建教育部重点实验室开放基金(No ZYNK201406)
关键词
补阳还五汤精简方
血管新生
核转录因子E2相关因子2
血红素加氧酶1-血浆Ⅷ因子相关抗原
微血管密度
thin recipe of Buyang Huanwu Decoction
angiogenesis
nuclear factor-erythroid 2 related factor 2(Nrf2)
heme oxygenase-1(HO-1)
von willebrand factor related antigen(v WF)
microvessel density(MVD)
