摘要
目的:探讨大肠癌患者人巨细胞病毒(human cytomegalovirus,HCMV)感染情况及其临床相关性.方法:采用化学发光(CLIA)法对大肠癌组(n=60)、大肠息肉组(n=60)及健康对照组(n=60)的外周血清进行HCMV免疫球蛋白G(immunoglobulin G,Ig G)、Ig M抗体检测;巢式PCR技术结合原位杂交方法检测32例大肠癌组织及癌旁正常肠黏膜组织中HCMV-UL138基因表达;用Fisher确切概率法比较两组间阳性率,均数比较采用t检验或单因素方差分析.P<0.05为差异有统计学意义.结果:大肠癌组、大肠息肉组、健康对照组血清H C M V-I g G阳性率为分别为95.0%(57/60)、98.3%(59/60)、96.7%(58/60);血清HCMV-Ig M阳性率分别为5.0%(3/60)、1.7%(1/60)、1.7%(1/60),3组血清H C M VIg G、Ig M差异无统计学意义(P>0.05).32例大肠癌组织巢式PCR检测HCMV-UL138阳性率为65.6%(19/32),相应癌旁正常肠黏膜组织中为12.5%(4/32),原位杂交法检测癌组织HCMV-UL138阳性率为62.5%(20/32),癌旁正常肠黏膜组织中为9.4%(3/32),大肠癌组织HCMV阳性检出率较癌旁正常组织显著升高(P<0.01).未发现HCMV感染与大肠癌患者的年龄、性别、肿块位置、肿块大小、组织病理分化类型、转移及Dukes分期有显著性关联.结论:大肠癌组织中存在HCMV感染,且H C M V相较于癌旁正常组织更倾向感染癌灶,提示HCMV感染可能参与大肠癌的发生发展过程.
AIM： To investigate the clinical relevance between human cytomegalovirus（HCMV） infection and colorectal cancer.METHODS： The specific immunoglobulin G（Ig G） and Ig M antibodies against HCMV in sera of colorectal cancer patients（n = 60）, patients with colorectal polyps（n = 60） and health controls（n = 60） were detected by chemiluminescence immunoassay. HCMV infection in colorectal cancer tissues and corresponding adjacent normal samples were determined through the detection of UL138 gene by nested PCR and in situ hybridization. The relationships between HCMV infection and clinical features of colorectal cancer were analyzed. RESULTS： The positive rates of HCMV-IgG in the colorectal cancer group, colorectal polyps group and healthy control group were 95.0%（57/60）, 98.3%（59/60）, and 96.7%（58/60）, respectively; the positive rates of HCMVIg M were 5.0%（3/60）, 1.7%（1/60） and 1.7%（1/60）, respectively. There were no statistically significant differences in the positive rates of HCMV-Ig G and HCMV-Ig M among the three groups. However, there was a signif icantdifference between colorectal cancer tissues and corresponding normal tissues in HCMVUL138 detection. The positive rates of HCMVUL138 were 65.6%（19/32） and 62.5%（20/32） in colorectal cancer tissues samples, and 12.5%（4/32） and 9.4%（3/32） in corresponding normal tissues as revealed by nested PCR detection and in situ hybridization, respectively. HCMV infection had no significant association with age, gender, location of mass, tumor size, histological differentiation, metastasis or Dukes stage.CONCLUSION： HCMV infection is associated with colorectal cancer. Compared with correspond ingnormalepi the lium, the neoplastic epithelium may be preferentially infected by the HCMV. This research suggests that HCMV infection may play a role in the occurrence and development of colorectal cancer.
出处
《世界华人消化杂志》
CAS
2016年第1期44-50,共7页
World Chinese Journal of Digestology
基金
浙江省自然科学基金资助项目
No.LY15H160059
浙江省医药卫生科技基金资助项目
No.2016KYB192
温州市科技计划项目
No.Y20140691~~
关键词
大肠癌
大肠息肉
人巨细胞病毒
UL138
临床病理特征
Colorectal cancer
Colorectal polyps
Human cytomegalovirus
UL138
Clinicopathological feature
