摘要
目的:分析口腔白斑(OLK)组织中树突状细胞的特征性表型。方法:应用免疫组化SP法检测10例正常黏膜、10例单纯增生白斑和20例异常增生白斑中树突状细胞特异性标记CD1a、CD209、CD123、CD83的表达状况,并进行统计学分析。结果:3组样本中CD1a、CD209均有表达。固有层内CD1a阳性郎格汉斯细胞(LCs)数量随病变程度逐渐升高,且异常增生白斑组高于单纯增生白斑组及正常黏膜组(P<0.01)。异常增生白斑及单纯增生白斑中CD209阳性DCs被大量召集,其数量显著高于正常黏膜。正常黏膜中未见CD123及CD83表达,但在单纯增生及异常增生白斑中CD123阳性和CD83阳性细胞数逐渐升高(P<0.01)。结论:朗格汉斯细胞、间质DC及浆细胞样树突细胞在OLK间质的表达水平增加逐渐成熟与机体的免疫监视功能密切相关,在OLK发展过程中均可能起到重要作用。
Objective： To investigate the functional status of dendritic cells（DCs） in oral leukoplakia（OLK） tissues. Methods： The expression of DC-specific markers CDla, CD209, CD123 and CD83 in 20 cases of OLK with abnormal dysplasia, 10 with simple dys- plasia and 10 of normal oral mucosa tissues was examined by immunohistochemistry. Results： CDla and CD209 positive DCs were found in all cases. More CDla positive Langehans cells（LCs） in lamina propria were found in OLK with abnormal dysplasia than in normal o- ral mucosa and OLK with simple dysplasia（ P 〈 0.01 ）. A great mount of CD209 positive stromal DCs were recruited in OLK. There was no CD83 positive and CD123 positive cell in normal oral mucosa, however, CD83 positive mature DCs and CD123 positive plasmacytoid DCs（PDCs） were observed in OLK（ P 〈 0.01 ）. Conclusion： OLK is characterized by the recruitment of different subsets of DCs, the different DC subsets may play an important role in the development of OLK.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2016年第1期67-71,共5页
Journal of Practical Stomatology
基金
广东省自然科学基金(编号:S2012010010382)
深圳市创新委国际合作(编号:ZYA201106100080A)
深圳市创新委基础研究(编号:JCY20130402114702120)
