摘要
目的:建立放射照射后牵张下颌骨成骨犬实验动物模型。方法:选取成年中国犬12只,实验组10只以60Co颊舌向照射下颌骨后部标定区域,照射方法为22.8 Gy、5.7 Gy/次,共4次(生物等效剂量为50 Gy/25次)。对照组2只不照射。照射完成后3个月,在动物下颌第五和第六臼齿间行骨皮质切开术,植入骨牵张器,经过1周的延迟期,2次/d,每次0.5 mm的速率连续牵张下颌骨10 d,然后固定8周。处死动物,以放射学,组织学和SPECT方法对牵张区新骨进行检查,对下牙槽神经进行组织学检查。结果:除实验组1只动物因麻醉意外死亡,其他动物都完成了实验。实验组和对照组新骨形成无明显差异。SPECT显示实验组成骨活跃。观察到下牙槽神经修复性组织学变化。结论:放射照射后牵张犬下颌骨可形成新骨。
Objective： To establish an animal model of bone distraction in the irradiated dog mandible. Methods： 10 Chinese dogs were used. 8 dogs received a unilateral irradiation of 60Co（ Group R） in the mandible with a total dose of 22.8 Gy in four 5.7 Gy frac- tions （ biologically equivalent to 50 Gy/25 fractions）. The other 2 dogs without irradiation served as the controls （ Group C ）. Bilateral corticotomy was made 3 months after completion of irradiation. After a 1- week latency period, bone distraction was activated at a rate of 0.5 mm twice daily for 10 days, followed by a consolidation phase of 8 weeks. New bone was evaluated by radiographic, histological and single photon emission computed tomography（SPECT） analysis. Results： One dog was excluded from the study due to anaesthetic death in group R. After 8 weeks of consolidation, no difference was found between the percent area of new bone of both groups. New bone was more mature and organized in Group C than that in Group R. SPECT analyses showed that there was active osteogenic activity in Group R. Conclusion： Distraction osteogenesis can be achieved in the irradiated dog mandible.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2016年第1期24-27,共4页
Journal of Practical Stomatology
关键词
放射照射
牵张成骨
动物模型
Irradiation
Distraction osteogenesis
Animal model
