摘要
目的 观察乌司他丁对脓毒症大鼠肠黏膜屏障功能的保护作用。方法采用盲肠结扎穿刺法制作脓毒症大鼠模型。健康Sprague—Dawley（SD）大鼠30只，随机分为假手术组（不进行盲肠结扎穿刺）、脓毒症组及乌司他丁组（各10只），术后及术后8h各组均给予10mL／kg等渗氯化钠溶液腹腔注射，乌司他丁组大鼠按照乌司他丁10×10^4U／kg的剂量加入等渗氯化钠溶液中腹腔注射。脓毒症造模成功后于术后0、8、12h抽取血液样本，采用酶联免疫吸附试验（ELISA）检测血浆降钙素原（PCT）、血清肠脂肪酸结合蛋白（iFABP）及二胺氧化酶（DAO）水平，并于术后12h麻醉后处死大鼠制作小肠组织样本，光镜下观察各组小肠组织病理学变化，Westernblot法检测肠道紧密连接蛋白表达活性的变化。结果假手术组小肠黏膜形态正常，各层解剖结构完整，绒毛排列整齐；脓毒症组肠壁明显变薄，黏膜萎缩，绒毛结构模糊，上皮坏死脱落，有大量中性粒细胞及嗜酸性粒细胞浸润；乌司他丁组小肠黏膜病理改变较脓毒症组明显减轻，肠壁结构尚完整，有黏膜水肿和少量中性粒细胞浸润。胞质紧密连接蛋白1（ZO一1）和闭锁蛋白表达活性假手术组最高，乌司他丁组次之，脓毒症组最低，且组间差异具有统计学意义（F=43．15和52．23，P〈0．05）。在术后0h，三组大鼠血浆的PCT、iFABP及DAO活性差异无统计学意义（F=11．17、22．45和13．58，P〉0．05）；在8h和12h脓毒症组及乌司他丁组各项指标均明显高于假手术组，同时脓毒症组又显著高于乌司他丁组，差异有统计学意义（R8h=85．26、44．59和101．47，F12h=59．44、49．26和69．57，P值均〈0．05）；组内各时间点比较，假手术组先升高后回落，脓毒症组持续升高，乌司他丁组先升高后持平。结论乌司他丁对脓毒症大鼠小肠黏膜和屏障功能有一定�
[Abstract] Objective To observe the protective effect of ulinastatin （UTI） on the intestinal barrier function of septic rats. Methods Septic rat model was established using Sprague-Dawley rats by cecal ligation and puncture （CLP） method. Thirty Sprague-Dawley rats were randomly divided into 3 groups （ n = 10 for each grop） ： sham group, septic group and UTI group. All rats received intraperitoneal injections of 0.9% saline （10 mL/kg） after and 8 h after surgery. In UTI group, UTI （ 10 x 104 U/kg in 10 mL/kg saline） was injected after and 8 h after surgery. Collect blood samples after 0, 8, 12 h after surgery to examine levels of procalcitonin （PCT）, intestinal fatty acid binding protein （iFABP） and diamine oxidase （DAO） by enzyme-linked immunosorbent assay （ELISA） method. Rats were killed 12 h after surgery to collect intestine tissue samples. Pathological changes of intestine were observed under microscopy, and the expression of tight junction protein-1 （ ZO-1 ） and occludin were analyzed by Western blot. Results In sham group, the mueosa structure was complete and the shape was normal, and villi stood neatly. In septic group, intestinal was expanded, intertinal mucosal was atrophic, villi were scanty. An inflammatory infiltrate with numerous nuetrophils was found in the mucosal. In UTI group, the level of severity was relatively slight. The relative optical density of Western blot images were decreased on ZO-1 and oecludin in CLP and UTI groups, and decreased more in CLP group （F =43.15 and 52.23, P 〈0.05）. At Oh after surgery, the plasma values of PCT, iFABP and DAO were similar in three groups （ F = 11.17, 22.45 and 13.58, P 〉 0. 05）. At 8h and 12h after surgery, values of PCT, iFABP and DAO in septic and UTI groups were nmch higher than those in the sham group, and those in UTI group were also significantly higher than those in septic group （Fs L, = 85.26, 44.59 and 101.47, F.2 h = 59.44, 49.26 and 69.57, all P 〈 0.05 ）. PCT, iFABP and DAO levels we
出处
《中华临床感染病杂志》
2015年第6期549-553,共5页
Chinese Journal of Clinical Infectious Diseases
基金
广东省自然科学基金项目（2015A030310735）
广东省省级科技计划项目（2014A020212651）
