摘要
为确定引起乌翅真鲨(Carcharhinus melanopterus)患病的病原种类及耐药情况,用常规方法从患病乌翅真鲨内脏取样分离得到的1株优势菌,通过回归感染试验确定该菌株的致病性,通过经典生理生化分析、API 20E细菌快速鉴定系统、16SrDNA序列分析对其进行鉴定,确定该株致病菌为弗氏柠檬酸杆菌(Citrobacter freundii)。药敏试验结果表明:该菌株对左氧氟沙星、头孢曲松钠、四环素、链霉素和庆大霉素等敏感。
This experiment was conducted to clarify pathogenic species and its drug resistance of isolated from the diseased blacktip reef shark Carcharhinus melanopterus.A dominant bacterial strain was isolated from the internal organ of the diseased shark.Pathogenicity of the isolated strain was determined by artificial infection.Bacterium was identified by traditional physiological and biochemical characteristics,API identification system and analysis of 16 SrDNA.The strain was identified as Citrobacter freundii.The susceptibility test to antibiotics demonstrated that the bacterial strain was susceptible to levofloxacin,ceftriaxone sodium,tetracycline,streptomycin and gentamicin.
出处
《海洋湖沼通报》
CSCD
北大核心
2015年第4期83-88,共6页
Transactions of Oceanology and Limnology
基金
北京市科学技术研究院萌芽计划(M2013-02)资助
关键词
乌翅真鲨
弗氏柠檬酸杆菌
分离鉴定
药敏试验
Blacktip reef shark
carcharhinus melanopteru
citrobacter freundii
isolation and identification
antibiotic sensitivity
