摘要
目的:研究局部稳定表达OPG在种植体周围骨再生修复过程中的作用。方法:将66只SD大鼠随机分为3组,A组种植体周围注射腺病毒Ad5-OPG-EGFP,B组种植体周围注射腺病毒Ad5-EGFP,C组种植体周围注射相同体积的PBS。在植入手术后1周、4周处死动物,行RT-PCR、组织学观察和Micro-CT扫描等检测,采用SPSS15.0软件包对相关数据进行t检验。结果:实验组hOPG表达量显著高于对照组(P<0.05),证实重组腺病毒载体Ad5-OPGEGFP建模成功,可顺利转染到骨组织中并稳定表达。植入术后4周,实验组和对照B组和C组大鼠均有新骨形成、包绕种植体。A组骨面积百分比(%BIC)、骨结合百分比(%BA)、标化后骨体积(BV/TV)、骨结合率(%OI)、单位体积内骨小梁数量(Tb.N)、单位体积内骨小梁厚度(Tb.Th)、骨小梁连接密度(Conn.D)均显著高于B组和C组(P<0.05),单位体积内骨小梁间隔(Tb.Sp)则显著低于B组和C组。结论:hOPG基因转染可在种植体周围稳定表达OPG,加快骨质再生,使种植体骨结合率增加。
PURPOSE： To study the role of local stable expression of OPG in peri-implant in bone regeneration during repair process. METHODS： Sixty-six SD rats were divided into A, B and C group randomly. In group A, adenovirus- hOPG-EGFP was injected around the implant; in group B, adenovirus-EGFP was injected around the implant; in group C, same volume of PBS was injected in the periphery of implant. The animals were sacrificed 1 week, 4 weeks after operation, and the femur bone containing the implant was collected. Micro-CT, H-E staining, toluidine blue staining were used to observe new bone formation. The data was analyzed with SPSS 15.0 software package. RESULTS： A successful vector of reconstructed adenovirus- OPG-GFP as gene therapy was established. In vitro the GFP was successfully expressed in the BMSCs of SD rats. At 4 weeks after implant surgery, osteointegration was observed in all groups. However, % BIC, % bone area, BV/TV, % OI, Th.N, Tb.Th, Conn.D in the peri-implant area in group A significantly increased compared with the control group （B and C）（P〈0.05）. Tb.Sp was also significantly less in experimental group （A） than that in control group （B and C）. CONCLUSIONS： Local hOPG transfection can be successfully conducted, and the stable expression of protein OPG in peri-implant area can remarkably promote osteogenesis.
出处
《中国口腔颌面外科杂志》
CAS
2016年第1期1-7,共7页
China Journal of Oral and Maxillofacial Surgery
基金
四川省科技厅科技支撑计划(2015SZ0127,2014SZ0019-4)
关键词
种植体
腺病毒
骨保护素
骨结合
Implant
Adenovirus
Osteoprotegerin
Osteointegration
