摘要
为了获得高产凝乳酶菌株,以产凝乳酶解淀粉芽孢杆菌为出发菌株,采用紫外线和硫酸二乙酯进行诱变。诱变后筛选得了一个突变株L-6,凝乳活力为1419.7 SU/m L,比原始菌株提高了40.2%;水解活力降低了22.39%,凝乳活力与蛋白水解活力的比值为125.64,比出发菌株提高了81.32%。传代实验表明,该菌株具有良好的遗传稳定性。
To obtain an industrial strain with high milk- clotting activity,Bacillus amyloliquefaciens producing milkclotting enzyme was mutagenized with ultraviolet and diethyl sulfate. On the basis of multiple mutation,mutant DES-6 was screened.The milk- clotting activity of L-6 reached 1419.7 SU / m L,which was 40.2% higher than that of the parent strain,while the proteolytic activity was reduced by 22.39%. The ratio of milk- clotting activity to proteolytic activity reached 125.64,which was 81.32% higher than that of the parent strain.The pass- generation test indicated that DES-6 had good genetic stability.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第3期156-159,共4页
Science and Technology of Food Industry
基金
国家自然科学基金项目(31560442)
“十二五”农村领域国家科技计划项目(2011AA100903)
甘肃省农业科技创新项目(GNCX-2014-31)
关键词
解淀粉芽孢杆菌
凝乳酶
诱变
Bacillus amyloliquefaciens
milk-clotting enzyme
mutation
