摘要
从水稻日本晴幼叶中提取RNA,以反转产物c DNA为模板PCR扩增得到了细胞分裂素氧化酶基因(CKO),酶切连接到p ET-28a载体上,构建了重组载体p ET-28a-CKO,在大肠杆菌BL21中表达了细胞分裂素氧化酶.
The total RNA from var nipponbare was extracted and the cytokinin oxidase gene（ CKO） was amplified by PCR using the reverse product c DNA as template,then CKO was ligated to vector p ET-28 a after restriction and a recombinant plasmid p ET 28a-CKO was constructed. Finally the rice cytokinin oxidase was expressed in E. coli BL21. The results showed that the recombinant plasmid p ET-28a-CKO was successfully constructed and the prokaryotic expression of rice cytokinin oxidase was achieved.
出处
《中南民族大学学报（自然科学版）》
CAS
北大核心
2015年第4期37-40,共4页
Journal of South-Central University for Nationalities：Natural Science Edition
基金
湖北省自然基金资助项目(2014CFB381)
中央高校自然科学基金资助项目(CZW14008)
