摘要
目的:研究不同浸种下维吾尔药材刺山柑种子萌发情况及幼苗酶活性,为提高刺山柑幼苗生产质量提供参考。方法:采用不同浸种条件即10%高锰酸钾浸种30 min、浓硫酸浸种30 min、100℃热水浸种30 min、75%乙醇浸种30 min对刺山柑种子进行发芽试验,测定种子的发芽率、发芽势、发芽指数、活力指数、胚根长度、幼苗鲜叶中的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性及10株植株烘干后的干物质质量,并与未浸种处理的对照组比较。结果:与对照组比较,各条件下以浓硫酸浸种30min后种子的发芽率、发芽势、发芽指数、活力指数、胚根长度值最高(P<0.05);且该条件下幼苗鲜叶中SOD、POD活性和干物质质量也相对较高。结论:以浓硫酸浸种30 min为刺山柑种子的较优浸种条件。此结果可为人工种植刺山柑药材提供依据。
OBJECTIVE：To study the germination and seedling enzymatic activity of Uygur medicine Capparis spirrosa seeds,and to provide reference for improving the quality of seedlings. METHODS：The germination test was carried out under different soaking conditions,i.e. 10% potassium permanganate for 30 min,concentrated sulfuric acid for 30 min,100 ℃ hot water for 30 min,75% alcohol for 30 min. The germination percentage,germination potential,germination index,activity index and length of radicel in seeds,the activity of SOD and POD in fresh leaves,were all determined,as well as the weight of 10 plants after dried. Those indicators were compared with those of control group（no soaking treatment）. RESULTS：Compared with control group,germination percentage,germination potential,germination index,activity index and length of radicel of seeds soaked in concentrated sulfuric acid for 30 min were the highest（P〈0.05）;the activity of SOD and POD in fresh leaves of seedlings and the weight of dried substance were also in high level. CONCLUSIONS：It can promote the best soaking condition of C. spirrosa seeds is to soak seed in concentrated sulfuric acid for 30 min. The trial result provides reference for the C. spirrosa artificial cultivation.
出处
《中国药房》
CAS
北大核心
2016年第4期566-568,共3页
China Pharmacy
关键词
刺山柑
种子
浸种条件
萌发
生长指标
酶活性
Capparis spinosa
Seed
Soaking condition
Germination
Growth indicator
Enzymes activity
