摘要
为研究社会隔离能否导致雌性啮齿动物产生焦虑,将成年雌性大鼠分别进行单独隔离饲养(单养)和四只一笼群养,十周后进行行为学和生理测试。行为结果显示:与群养组相比,单养组大鼠在旷场中的直立次数减少,明暗箱测试中的暗箱停留时间增长,穿越次数减少;说明长期社会隔离使雌鼠产生了焦虑。虽然两组大鼠与应激和生殖相关的生理器官重量没有差异,但单养组血清皮质醇和孕酮水平比群养组低,说明长期社会隔离导致雌鼠下丘脑-垂体-肾上腺轴和下丘脑-垂体-性腺轴的调节异常,可能会对雌鼠的生殖生理带来不利影响。
To solve the problem that whether long-term isolation would lead to anxiety in rodents. The effects of long-term social isolation on behavior and physiology in female rats were studied. In the study, female rats were divided into two groups, isolation-reared group and socially-reared group. In isolation- reared group, rats were individually reared in one cage. In socially-reared group, rats were housed in groups of four. Behavioral and physiological index were tested ten weeks later. The following results were obtained. Compared to the socially-reared group, isolation-reared group showed less rearing in open field test, and the more time female rats spent in the dark box, the less cross these rats in light/dark box, which suggested that chronic isolation induced anxious responses in female rats. Although relative weight of adrenals, spleen, ovaries and uteri did not differ between the two groups, the serum level of cortisol and progesterone were lower in socially-reared group. It is suggested that the dysregulations of hypothalamic-pituitary-adrenal axis and hypothalamic-pituitary-gonadal axis is caused by long-term socialisolation, which may impart a deleterious effect on reproductive physiology in female rats.
出处
《陕西师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第1期71-77,共7页
Journal of Shaanxi Normal University：Natural Science Edition
基金
国家自然科学基金(31272322)
河南省高等学校重点科研项目(15A180053)
农业虫害鼠害综合治理研究国家重点实验室开放课题(ChineseIPM1403)
南阳师范学院校级项目(Zx2014063)
关键词
社群隔离
雌性大鼠
焦虑行为
血液激素
生理指标
social isolation
female rats
anxiety behavior
serum hormone
physiology index
