摘要
目的探讨槲皮素对高脂膳食诱导动脉粥样硬化(AS)小鼠自噬相关蛋白表达的影响。方法采用高脂高胆固醇膳食喂养apo E-/-小鼠24 w,建立AS模型,同时给予三个剂量槲皮素干预。饲养结束后处死动物,分离主动脉,用油红O染色检测主动脉脂质沉积;用Western印迹方法检测主动脉组织中雷帕霉素靶蛋白(m TOR)、p62和溶酶体相关膜蛋白2(LAMP-2)的蛋白表达变化。结果与模型组小鼠相比,100 mg/kg体重的槲皮素干预可显著减少(P<0.05)小鼠主动脉脂质浸润并缓解AS损伤,而50 mg/kg体重和100 mg/kg体重槲皮素干预均能使AS小鼠主动脉组织中m TOR、p62和LAMP-2蛋白表达显著下调(P<0.05)。结论高脂膳食诱导的小鼠自噬功能损伤可能是AS的发病机制之一,槲皮素可通过自噬调节功能缓解AS损伤。
Objective To investigate protective role of quercetin against atherosclerosis via autophagy regulation in high-fat diets mice. Methods ApoE^-/- mice were given high-fat diets and（or） three different doses of quercetin for 24 weeks. All the animals were sacririced at the end of feeding, and the aortas were removed for red oil O staining in order to analyze the atherosclerotic lesions. The protein ex- pressions of mTOR, p62 and LAMP-2 in aortic tissues were measured with Western blot. Results The atherosclerotic lesions were alleviated and lipid infiltration was decreased in aortic（ P〈0.05 ） and quercetin-treated group （100 mg·kg^-1·bw^-1 ） as compared to high-fat diets group. The expressions of roTOR, 1362 and LAMP-2 were signiricantly （ P〈0.05 ） reduced at translational level in quercetin-treated groups （ 50 and 100mg·kg^-1·bw^-1）. Conclusions Autophagy dysfunction is the potential mechanism of atherosclerosis, and quercetin has the significant protective effects by autophagy regulation and alleviating atheroscleroticiesions.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2016年第3期539-542,共4页
Chinese Journal of Gerontology
基金
国家自然科学基金项目(31201351
81172658)
