摘要
目的研究奥氮平与氟西汀单独或两药联合给药对大鼠脑内柠檬酸合成酶活性短期和长期的影响。方法 135只Wistar大鼠随机分为对照组与实验组。对照组腹腔注射生理盐水,实验组再分为几个亚组。2个剂量奥氮平组(3,6 mg·kg^(-1)),2个剂量氟西汀组(12.5,25.0 mg·kg^(-1)),联合用药组:A组(3 mg·kg^(-1)奥氮平+12.5 mg·kg^(-1)氟西汀)、B组(3 mg·kg^(-1)奥氮平+25.0 mg·kg^(-1)氟西汀)、C组(6 mg·kg^(-1)奥氮平+12.5 mg·kg^(-1)氟西汀)、D组(6 mg·kg^(-1)奥氮平+25.0 mg·kg^(-1)氟西汀),连续给药28 d。用分光光度法测定并比较第1次给药后2 h、末次给药后2,24 h的大鼠前额叶皮层、海马区和纹状体柠檬酸合成酶的活性。结果与对照组相比,在给药第1次后2 h,2个剂量奥氮平组、大剂量氟西汀组、联合A组的大鼠海马区柠檬酸合成酶活性明显增加(P<0.05)。结论短程小剂量奥氮平联合氟西汀可显著增加大鼠脑内柠檬酸合成酶活性。
Objective To evaluated the effect of acute and chronic ad- ministration of fluoxetine, olanZapine and the combination of fluoxetine/ olanzgtine on citrate synthase activity in rat brain. Methods One hun- dred and thirty - five Wistar rats were randomly divided into control group and experimental group. The rats of the control group received injections of saline. The rats of the experimental group were divided into 8 sub groups by the ways of treament：low and high dose of olanzapine groups （3,6 mg· kg-1 ） ,the low and high dose of fluoxetine groups（ 12.5,25.0 mg· kg- 1 ） , the two - drug combination ： A group （ 3 mg· kg- 1 olanza- pine ＋ 12. 5mg· kg-1 fluoxetine）, B group（3 mg· kg-1 olanzapine ＋ 25 mg, kg-1 fluoxetine）,＇C group （6 mg· kg-1 olanzapine ＋ 12.5 mg· kg-1 fluoxetine）, D group（6 mg· kg-1 olanzapine ＋25 mg ＇ kg-1 fluoxetine）. Saline or mediations were given once a day, which lasted for 28 d. Citrate synthase activity in prefrontal cortex, hippocampus and striatum of the rats in control and experimental groups were detected 2 h after the first dose and 2 h, 24 h after the last dose. Results Compared with eontral group, the citrate synthase activity increased significantly in two doses of olanzapine groups, the large doses fluoxetine group and the combination A group at the time 2 h after first administration （P 〈 0. 05 ）.Conclusion Short - term treatment with the combination of olanzapine and fluoxetine significantly increased citrate synthase activity in rat brain.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2016年第3期235-237,共3页
The Chinese Journal of Clinical Pharmacology
基金
浙江省医药卫生科技计划基金资助项目(2009A149)
关键词
奥氮平
氟西汀
短程
柠檬酸合成酶
olanzapine
fluoxetine
short - term
citrate synthase
