摘要
目的探讨血清可溶性CD40配体(sCD40L)在结直肠癌患者的早期诊断及患者预后评估中的价值。方法采用流式荧光技术和ELFA方法分别检测78例结直肠癌患者及95例健康对照者血清sCD40L和癌胚抗原(CEA)的浓度。ROC曲线比较sCD40L和CEA对结直肠癌诊断的临床价值。将结直肠癌患者分为sCD40L高表达组和低表达组,分析血清sCD40L的水平与临床病理参数的关系,以Kaplan-Meier生存曲线分析sCD40L的表达水平对患者预后的影响。结果结直肠癌患者血清sCD40L的浓度显著高于健康对照者;ROC曲线显示,sCD40L对结直肠癌诊断的特异度和敏感度优于CEA,sCD40L的AUC值>CEA。血清sCD40L的浓度高低与结直肠癌患者是否发生淋巴结转移、肿瘤浸润深度和TNM分期密切相关;sCD40L高表达组生存时间显著低于低表达组。15例在术后1年复查sCD40L,结果显示血清sCD40L浓度显著下降。结论 sCD40L对结直肠癌的早期诊断和患者预后评估具有一定价值。
Objective To investigate the clinical value of soluble CD40 ligand （sCD40L） in the early diagnosis of patients with colorectal cancer and their prognostic assessment. Methods The serum sCD40L and carcinoembryonic antigen （CEA） concentration were detected by Luminex xMAP analysis and ELFA method in 78 patients with colorectal cancer and 95 healthy control people. ROC curve was used to make comparison between the clinical value of sCD40L and CEA in the diagnosis of colorectal cancer. Those patients with colorectal cancer were divided into sCD40L high expression group （sCD40Lhigh） and sCD40L low expression group （sCD40Llow）. Analysis was made in the correlation between serum sCD40L level and clinical pathological parameters. Kaplan-Meier survival curve was used to analyze the effects of sCD40L expression level on patients prognosis.Results The serum sCD40L concentration of patients with colorectal cancer was significantly higher than that of healthy controls. ROC curve showed that the specificity and sensitivity of sCD40L in the diagnosis and prognosis of colorectal cancer was superior to those of CEA, and the AUC value of sCD40L was larger than that of CEA. The concentration of serum sCD40L was closely correlated with lymph node metastasis, the depth of tumor invasion, and TNM staging. The survival time of patients with high sCD40L expression level was significantly shorter than that of those in the low expression group. Reexamination showed the significant decreasing of serum sCD40L concentration in 15 cases one year after the operation. Conclusion SCD40L has certain value in the evaluation of the early diagnosis and prognosis of patients with colorectal cancer.
出处
《西南国防医药》
CAS
2016年第1期47-50,共4页
Medical Journal of National Defending Forces in Southwest China
