摘要
目的:探讨胡桃醌对人卵巢癌SKOV3细胞的毒性作用及机制。方法:采用MTT法检测胡桃醌对卵巢癌SKOV3细胞生长的作用;流式细胞术检测胡桃醌对卵巢癌SKOV3细胞凋亡的影响;采用DCF-DA染色荧光显微镜观察细胞内的活性氧簇(ROS)水平;采用Western blot检测卵巢癌SKOV3细胞细胞色素C(Cyt C)和活化caspase-3的水平。结果:与对照组比较,胡桃醌对SKOV3细胞的生长有明显抑制作用,且抑制作用随药物浓度和时间的增加而增强(P<0.05)。流式细胞术检测50μmol/L胡桃醌作用细胞24 h和48 h后诱导细胞凋亡,早期凋亡率和晚期凋亡率均高于对照组(P<0.01)。胡桃醌显著提高细胞内的ROS水平,上调细胞Cyt C和活化caspase-3的蛋白水平。结论:胡桃醌通过促进SKOV3细胞ROS的积累诱导细胞凋亡和抑制细胞生长。
AIM： To study the cytotoxicity of juglone on ovarian cancer SKOV3 cells. METHODS： The activity of SKOV3 cells was detected by MTT assay. The cells apoptosis was examined by flow cytometry. The level of reactive oxygen species（ ROS） was measured by DCF-DA staining. The protein levels of cytochrome C（ Cyt C） and activated caspase-3 were evaluated by Western blot. RESULTS： MTT assay showed that juglone significantly inhibited the growth of SKOV3 cells in dose- and time-dependent manners（ P 0. 05）. The early apoptotic rate and late apoptotic rate of SKOV3 cells in 50 μmol / L juglone group at 24 and 48 h were higher than those in control group（ P 0. 01）. Moreover,juglone induced ROS accumulation,and increased the protein levels of Cyt C and activated caspase-3. CONCLUSION： Juglone inhibits the cell growth and induces the apoptosis of SKOV3 cells by ROS accumulation.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2016年第1期156-159,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81202031)
吉林市科技局基金资助项目(No.2013625028)
关键词
胡桃醌
卵巢癌
活性氧簇
细胞凋亡
Juglone
Ovarian cancer
Reactive oxygen species
Apoptosis
