摘要
目的:探讨胰岛素样生长因子结合蛋白3(IGFBP-3)基因多态性和青春期女子体操运动员骨生长代谢的关联性。方法:对62名青春期汉族女子体操运动员进行观察研究,测试其2013年3月25日的骨量、骨密度、胰岛素样生长因子1(IGF-1)、IGFBP-3、骨钙蛋白、骨碱性磷酸酶、25-羟维生素D。采用haploview软件及hapmap、NCBI数据库选取IGFBP-3基因多态位点,应用SNa Pshot SNP分型方法进行基因分型。结果:IGFBP-3基因多态位点rs2854744、rs2270628、rs2854746、rs3110697、rs2453839均符合Hardy-Weinberg遗传定律。女子体操运动员IGFBP-3基因rs2270628位点与各位点骨量及骨密度关联。rs2270628CC基因型大腿骨矿含量、全身骨矿含量、上肢骨密度、大腿骨密度、脊柱骨密度、全身骨密度显著高于CT+TT基因型。IGFBP-3水平与rs2453839多态性关联,TT基因型的IGFBP-3水平显著高于CT+CC型。结论:IGFBP-3基因多态位点rs2270628可作为青春期女子体操运动员骨生长代谢的分子遗传学标记。
Objective The purpose of this study is to determine the relation of insulin-like growth factor binding protein 3 gene（IGFBP-3）polymorphism to the bone growth and bone metabolism in pubertal female gymnasts. Methods The bone mass,body bone density,insulin-like growth factor（IGF-1）and IGFBP-3 of 62 adolescent female gymnasts were observed in this study. The Haploview software,and Hapmap and NCBI database were used to select single nucleotide polymorphisms locis. The genotypes at rs2854744,rs2270628,rs2854746,rs3110697,and rs2453839 were examined by SNa Pshot SNP. Results The genotype distributions at the 5 locis were accorded with the Hardy-Weinberg equilibrium（P 〉0.05）. The bone mass and body bone density were associated with SNP rs2270628 polymorphism. The femur bone mass,total body bone mass,upper limb bone mineral density,spine bone mineral density and whole body bone density in the gymnasts carrying CC genotype were significantly higher than the gymnasts carrying CT ＋TT genotype. The level of IGFBP-3 was associated with SNP rs2453839 polymorphism. The level of IGFBP-3 in the gymnasts carrying TT genotype was higher than those carrying CT＋CC genotype. Conclusion The SNP rs2270628 polymorphism in IGFBP-3 gene could be used as a molecular genetic marker to predict the bone growth and bone metabolism in pubertal female gymnasts.
出处
《中国运动医学杂志》
CAS
北大核心
2016年第1期45-52,共8页
Chinese Journal of Sports Medicine
基金
国家体育总局体育科学研究所基本科研业务费课题(1305)
