摘要
采用pH 2的柠檬酸溶液提取海带中的多糖,将海带多糖(polysaccharide from Laminaria japonica,LPA-P)通过Sevag法除蛋白、透析除盐处理、离子交换柱处理,分离纯化得到3种多糖组分LPA-P1、LPA-P2、LPA-P3,探讨了海带多糖的分子质量、硫酸基含量、单糖组成与其抗氧化能力和胆酸盐吸附能力的内在联系。结果表明:LPA-P及其纯化组分均含有硫酸基,且LPA-P3最多为5.36%,LPA-P1最少为1.22%;海带多糖及其纯化组分的单糖组成均以岩藻糖、半乳糖和甘露糖为主,其中LPA-P和LPA-P3主要的单糖为半乳糖,而LPA-P1和LPA-P2主要的单糖为甘露糖,海带多糖LPA-P3中岩藻糖含量最高;海带多糖LPA-P、LPA-P1、LPA-P2和LPA-P3的平均分子质量为20.13、17.55、28.71、17.14 k D。海带多糖的氧自由基吸收能力和2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐自由基(2,2’-azinobis(3-ethylbenzothi azoline-6-sulfonic acid)ammonium salt radical,ABTS^+·)清除能力从大到小依次为LPA-P3>LPA-P>LPA-P2>LPA-P1;LPA-P3具有最强的胆酸盐吸附能力,LPA-P和LPA-P2次之且无显著性差异,LPA-P1基本不具有胆酸盐吸附能力。结合LPA-P及其纯化组分的结构特性,推测高硫酸基含量、主要单糖组成为岩藻糖和半乳糖的海带多糖具有更强的胆酸盐吸附能力及抗氧化活性。
Polysaccharide from Laminaria japonica（LPA-P） was isolated by using aqueous citric acid solution. After the removal of protein and salt, LPA-P was purified by DEAE Sepharose fast flow chromatography to obtain LPA-P1, LPA-P2, and LPA-P3. The respective relationships of bile salt adsorption capacity and antioxidant activity with molecular weight, sulfated group content and monosaccharide composition for LPA-P and its fractions were investigated. The results indicated that all LPA-P and its purified fractions contained sulfated group. LPA-P3 had the highest sulfated group content of 5.36%, while LPA-P1 had the lowest of only 1.22%. The major monosaccharide composition consisted of fucose, galactose and mannose. Galactose was the major monosaccharide in both LPA-P and LPA-P3, whereas mannose dominated the monosaccharide composition of both LPA-P1 and LPA-P2. LPA-P3 was the highest in fucose. The average molecular weights of LPA-P, LPA-P1, LPA-P2 and LPA-P3 were estimated to be 20.13, 17.55, 28.71, and 17.14 k D, respectively. LPA-P3 exhibited the strongest antioxidant capacity but LPA-P1 the weakest; the antioxidant capacity of LPA-P was a little stronger than that of LPA-P2. LPA-P3 had the strongest bile salt adsorption capacity, followed by LPA-P and LPA-P2. LPA-P1 had substantially no bile salt adsorption capacity. According to the structure-activity relationship of polysacchrides from Laminaria japonica, it is speculated that the higher the contents of sulfated group as well as fucose and galactose, the stronger bile salt adsorption capacity and antioxidant activity of the polysaccharides.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第1期22-27,共6页
Food Science
基金
国家高技术研究发展计划(863计划)项目(2014AA093602)
中央高校基本科研业务费专项资金项目(2014ZZ0053)
关键词
海带
多糖
胆酸盐吸附能力
抗氧化活性
Laminaria japonica
polysaccharides
bile salt adsorption capacity
antioxidant activity
