摘要
目的:探讨新建成的体外受精(IVF)胚胎实验室挥发性有机化合物(volatile organic compound,VOC)的浓度变化及不同浓度VOC环境对小鼠体外受精和胚胎发育的影响,同时比较目前常用的去除实验室内VOC方法的效率。方法:测定胚胎实验室装修后不同时间段的VOC浓度变化,比较不同浓度的VOC对小鼠体外受精率、2-细胞率和囊胚率的影响;比较排风扇抽风机、Coda空气净化器和单纯活性炭包去除室内VOC的效率。结果:装修后第1个月(M1)VOC浓度为384.00 ppb,显著高于对照组(199.00 ppb)(P<0.05);装修后第2个月(M2)VOC浓度为279.50 ppb,与对照组相比差异具有统计学意义(P<0.05);装修后第3个月(M3)VOC浓度为210.25 ppb,与对照组(206.00 ppb)相比差异无统计学意义(P>0.05)。装修后1个月(M1)实验组体外受精率、2-细胞率和囊胚率分别为62.3%、71.5%、72.0%,显著低于对照组(91.8%、92.2%、93.0%),组间差异具有统计学意义(P<0.05)。装修后3个月(M3)时受精率、2-细胞率和囊胚率(91.5%、90.7%、91.4%)与对照组(89.8%、90.1%、92.5%)相比差异无统计学意义(P>0.05)。Coda空气净化器处理3个月后可去除室内60.15%的VOC,显著高于排风扇抽风(51.88%)和单纯活性炭吸附(28.95%)的去除效率。结论:实验室内高浓度的VOC会对小鼠体外受精胚胎造成严重的胚胎毒性,导致受精率、2-细胞率和囊胚率降低,胚胎质量下降和发育受阻;Coda空气过滤器是一种有效的去除实验室内VOC的方法。
Objective： To explore the concentration changes of volatile organic compounds （VOC） in newly constructed in vitro fertilization （IVF） embryo laboratories and their effects on the IVF and embryo development of mice. At the same time, the efficiency of different methods to remove VOC in laboratories was compared. Methods： The changes of VOC concentration in decorated embryonic laboratories were measured at different time periods, and the effects of various VOC concentrations on the IVF rate, 2-cell rate and blastocyst rate of mice were analyzed. The removal rates of indoor VOC of drawing fan, coda air purifiers and small packaged carbon were compared. Results： One month after decoration （M 1）, VOC concentration was 384.00 ppb, which was significantly higher than that of the control （199.00 ppb, P〈0.05）. It decreased to 279.50 ppb 2 months later （M2）, but it was still significantly higher than that of the control （P〈0.05）. VOC concentration was not significantly different from that of the control 3 months after the decoration （210.25 ppb vs 206.00 ppb）. The IVF rate, 2-cell rate and blastocyst rate one month after decoration （M1） were 62.3%, 71.5% and 72.0%, respectively, which were significantly lower than those of the control （91.8%, 92.2%, 93.0%） （P〈0.05）. The IVF rate, 2-cell rate and blastocyst rate 3 months after the decoration （M3） were 91.5%, 90.7% and 91.4%, respectively, which were not significantly different from that of the control （89.8%, 90.1% and 92.5%, P〉0.05）. The clearance efficiency of indoor VOC after 3 months by Coda air purifiers was 60.15%, which was remarkably higher than that of drawing fan （51.88%） and activated carbon package （28.95%）. Conclusion： High concentration of VOC in laboratories can cause serious toxicity of the mouse embryos developed in vitro, including the decrease of fertilization rate, cleavage rate and blastocyst rate. Coda air filter is an effective device to remove VOC in the laboratory.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2016年第1期15-20,共6页
Reproduction and Contraception
