摘要
以大豆品种合丰25和Bayfield为材料,克隆大豆2-甲基-6-植基-1,4-苯醌甲基转移酶(MPBQMTase)基因,获得1 029 bp的MPBQ-MT基因c DNA序列。通过序列比对发现10个大豆MPBQ-MT基因c DNA区内的潜在SNP位点。采用生物信息学方法分析MPBQ-MT基因编码的氨基酸序列和蛋白质结构。结果表明:MPBQ-MT基因CDS区共编码342个氨基酸,其中缬氨酸(Val)含量最多,占该基因编码氨基酸总数的8.8%;半胱氨酸(Cys)含量最少,占该基因编码氨基酸总数的1.2%;MPBQ-MT蛋白N端包含由58个氨基酸组成的叶绿体转运肽,为亲水性蛋白质。合丰25与Bayfield的MPBQ-MT蛋白质二级结构有所不同,2个品种的MPBQ-MT蛋白质中α-螺旋分别约占25.44%和27.49%,β-转角分别约占9.36%和9.06%,无规则卷曲分别约占40.35%和38.60%。2个品种的MPBQMT蛋白质中片层结构均约占24.85%。两个品种的MPBQ-MT蛋白质均有1个S-腺苷基甲硫氨酸结合位点。对MPBQ-MT蛋白三级结构进行预测,发现去掉转运肽后,MPBQ-MT蛋白质三级结构主要由11个连续的α-螺旋区域和8个连续的片层结构区域组成。结合MPBQ-MT蛋白三级结构对MPBQ-MT基因的生物学功能进一步分析,提出了较为合理的MPBQ-MT作用模型。由系统发生树可知MPBQ-MT蛋白在大豆与菜豆中的亲缘关系较近。
Cloned the 2-methyl-6-phytyl-1,4-benzoquinone methyltransferase （MPBQMTase） of soybean and obtained the cDNA encoding MPBQMTase using Hefeng 25 and Bayfield as materials. The cDNA sequence analysis showed that the cDNA was 1 029 bp in length.Ten latent SNPs were found in CDS of MPBQ-MT gene in soybean by sequences blast.The amino acid sequence and protein structure of MPBQ-MT gene were analyzed by bioinformatics method. The result showed that the CDS region of MPBQ-MT gene encoded 342 amino acid residues with the most content of Valine which accounted for 8.8% of the total amino acids and the least content of Cysteine which accounted for 1.2% of the total amino acids N-terminal of MPBQ-MT protein contains chloroplast transit peptide composed by 58 terminal amino acids and MPBQ-MT was hydrophilic protein. The secondary structure of MPBQ-MT protein was different in Hefeng 25 and Bayfield.About 25.44% and 27.49% of the protein sequence were alpha helixes respectively in the two varieties, about 9.36% and 9.06% of the protein sequence were beta turn, 40.35% and 38.60% of the protein sequence were random coil 24.85% of the protein sequence were lamellar structure in both varieties. S-adenosyl methionine binding sites exist in MPBQ-MT protein of both varieties.The three stage structure analysis showed 11 continuous alpha helixes and 8 continuous film layer structure exist in MPBQ-MT protein. The model of MPBQ-MT was obtained based on the three stage structure of MPBQ-MT protein and biological function analysis of MPBQ-MT. Phylogenetic tree indicated the relationship of MPBQ-MT protein was close in soybean and bean.
出处
《大豆科学》
CAS
CSCD
北大核心
2016年第1期39-45,共7页
Soybean Science
基金
中国博士后项目(20110491025)
东北农业大学博士启动基金(2012RCB32)
