摘要
目的探究富含n-3多不饱和脂肪酸(polyunsaturated fatty acid,PUFA)的苏子油高脂饮食对胰岛素抵抗大鼠胰岛素敏感性及其相关基因表达的影响。方法将胰岛素抵抗模型大鼠随机分为2组:高脂组(high fat group,HF)和苏子油组(perilla oil group,PO),PO为苏子油替代HF中猪油比例的20%,4周后测定大鼠胰岛素敏感性;气相色谱法检测苏子油及大鼠血清α-亚麻酸(α-linolenic acid,ALA)含量;Western blot方法检测骨骼肌葡萄糖转运蛋白4(glucose transporter 4,GLUT4)和胰岛素受体底物-1(insulin receptor substrate-1,IRS-1)蛋白表达;real time PCR方法检测骨骼肌Glut4、Irs-1 mRNA表达。结果苏子油干预4周后,与HF组相比,随着PO组大鼠ALA摄入量及血清ALA含量升高,GLUT4蛋白和mRNA表达量均显著升高,而IRS-1蛋白和mRNA表达均显著降低;高胰岛素-正常血糖钳夹实验结果显示两组大鼠胰岛素敏感性没有明显差异。结论 ALA(0.556 g/d)高脂饮食干预可以调节骨骼肌GLUT4、IRS-1表达,但不能改善胰岛素抵抗大鼠胰岛素敏感性。
Objective This study aims to investigate the effects of high-fat diet rich in perilla oil on the insulin sensitivity-related gene expression in skeletal muscle in insulin resistant rats. Methods The insulin resistant（ IR） rat models were randomly divided into 2 groups,including high fat group（ HF） and perilla oil（ PO） intervention group fed with 20% substitution of lard energy in the HF. The insulin sensitivity of rats was measured after 4 weeks. The α-linolenic acid（ ALA） content of PO in the rat plasma were analyzed by gas chromatograph. Real-time PCR was applied to measure glucose transporter 4（ GLUT4） and insulin receptor substrate-1（ IRS-1） mRNA,and Western blot assay was used for detecting the expression of GLUT4 and IRS-1 in the skeletal muscle. Results At the gene and protein levels,PO remarkably reduced the level of IRS-1 and upregulated the level of GLUT4 with increasing intake of ALA and serum ALA content in IR rats. The results of hyperinsulinemic-euglycemic clamp test showed no significant difference between the two groups. Conclusions The results of our study suggest that consumption of n-3 PUFA at levels that can typically be foundin the diet fed to IR rats in the form of ALA（ 0. 556 g / d） may not improve insulin sensitivity,even though regulating the expression of GLUT4 and IRS-1 in the skeletal muscle.
出处
《中国比较医学杂志》
CAS
北大核心
2015年第12期1-5,共5页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金面上项目(NO.31272386)
