摘要
目的研究枳椇子总黄酮的超声提取最佳工艺条件;方法利用超声提取法,以药材目数、料液比、超声温度、超声功率为考察因素,枳椇子中芦丁的含量为考察指标,紫外分光光度法测定（417nm）结果最佳提取工艺：干燥药材（20-40目）中加入14倍量的体积分数70%乙醇溶液,超声功率：160 W、温度：70℃、时间：60min时含量最高,为1.17mg/g。回归方程为A=0.033 3C-0.022 3（r=0.999 3）,芦丁在5.84-23.36μg/mL范围内线性关系良好,平均回收率为96.92%。结论超声法提取枳椇子中的芦丁优势明显,有效成分溶出快、用时短、效率高,紫外测定其含量灵明度较高、准确。
Objective to optimize the extracting method of total flavonoids from Turnjujube Methods extracting total flavonoids from Turnjujube by ultrasonication extraction,considering the size of raw material,material-toliquid ratio,extraction temperature,extraction frequency as affecting factor by L9（34）orthogonal design;using rutimun as a reference substance,the rutimun in Turnjujube was determined by UV absorption wave at 417 nm.Results the best extraction condition by ultrasonication extraction was to use 70%alcohol at 70℃for 1hours with160 W,material-to-liquid ratio 20-40mesh granule,and the resultant total flavonoids yield was 1.17mg/g,equation of regression：A =0.033 3C-0.022 3（r =0.999 3）,the linearity of rutimun was obtained with in the range of5.84-23.36μg/mL,the average recovery was 96.92%.Conclusion the method is convenient and reliable,this result provide a suitable method research.
出处
《河南大学学报（医学版）》
CAS
2015年第4期247-251,共5页
Journal of Henan University:Medical Science
关键词
枳椇子
总黄酮
芦丁
超声提取
Hovenia dulcisThunb
Total flavonoids
Rutin
Ultrasonic extraction
