摘要
从患病凡纳滨对虾(Litopenaeus vannamei)体内提取白斑综合征病毒(WSSV),PCR证实WSSV毒性,将病毒粗提液稀释10-2、10-3、10-4和10-5倍,攻毒实验表明,最佳攻毒剂量为10-4倍稀释液。对凡纳滨对虾(Litopenaeus vannamei)分别注射0.05、0.10、0.15 mg/m L的溶藻弧菌(Vibrio alginolyticus)肽聚糖,免疫24 h后感染WSSV,测定其免疫保护率和肝胰腺的免疫学指标,探讨溶藻弧菌肽聚糖对凡纳滨对虾抗WSSV的影响。结果表明:对虾的保护率随着肽聚糖的浓度增大而增大,与对照组相比,0.05、0.10、0.15 mg/m L组的保护率分别为36.67%、46.67%、56.67%;实验组凡纳滨对虾肝胰腺中的酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)和一氧化氮合成酶(NOS)活力与对照组相比的差异有统计学意义(P<0.01)。溶藻弧菌肽聚糖可有效增强凡纳滨对虾自身的非特殊性免疫能力,提高抗WSSV的能力。
White Spot Syndrome Virus（WSSV） was extracted from Litopenaeus vannamei suffering from White Spot Syndrom, and WSSV toxicity was confirmed by PCR. The virus crude extract was diluted 10^（-2）, 10^（-3）, 10^（-4） and 10^（-5） times, respectively, and the best attack dose of 10^（-4） fold dilution was ascertained after challenge with WSSV. Peptidoglycan at the concentration of 0.05, 0.10 and 0.15 mg/m L was injected into L. vannamei, respectively. 24 hours later, WSSV were injected into shrimps, and the immune protection rate and the immunological indexes in hepatopancreas were measured to investigate the influence of Vibrio alginolyticus peptidoglycan in shrimps against WSSV. The results showed that the immune protection rate increased with the increase of peptidoglycan concentration, and they reached 36.67%, 46.67% and 56.67%, respectively, compared with the control group. The activities of acid phosphatase（ACP）, alkaline phosphatase（AKP）, superoxide dismutase（SOD） and nitrogen monoxide synthetic enzyme（NOS） in hepatopancreas had statistical differences from the control（P 0.05）. The V. alginolyticus peptidoglycan could effectively enhance the L. vannamei non-specific immunity and promise approaches to protect the shrimp from WSSV infection.
出处
《广东海洋大学学报》
CAS
2015年第6期40-46,共7页
Journal of Guangdong Ocean University
基金
广东省海洋渔业科技推广专项项目(A201100D03)
广东省海洋经济创新发展区域示范专项项目(GD2012-A03-012)
湛江市科技计划项目(2013A03021)
