摘要
运用传统形态分类学和现代分子系统学两种方法鉴定在内蒙古贺兰山国家级自然保护区内新发现的啮齿动物,通过传统形态分类学初步鉴定为东方田鼠,进一步利用分子系统学中的DNA条形码技术,通过细胞色素b基因(cytochrome b,cytb)序列,构建分析系统进化树。结果表明:所有比较的21种田鼠基因序列的cytb基因,大致可分为3组。本次试验个体与沼泽田鼠的亲缘关系最近,而沼泽田鼠即为东方田鼠的别称。且在NCBI中使用BLAST相似性检索进行物种序列比对,得到了可供最后比较分析的高质量序列各10条,最终发现与之同源性最高的鼠种为东方田鼠,从而确定本研究中物种为东方田鼠。
Using traditional morphological taxonomy and modern molecular biology,a new species of rodents which was found in the Inner Mongolia Helan Mountain National Nature Reserve was identified. The preliminary results of traditional morphological taxonomy indicated that it was Microtus fords,Furthermore,and built analysis system of the evolutionary tree by The sequence of cytochrome b gene using the DNA barcoding technology in the molecular biology. The results indicated that all gene sequence of ITS 1 coming from 21 compared voles species could be roughly divided into three groups. The sample and swamp voles were the closest relatives of voles,and swamp vole＇s nickname is the east vole. Using BLAST similarity retrieval for sequence alignment among species in NCBI,Ten high quality sequence for the final comparison analysis were obtained. Eventually,The eastvoles has the highest homology with tested sample,thus determining the species in this study was Microtus fords.
出处
《经济动物学报》
CAS
2015年第4期195-201,共7页
Journal of Economic Animal
基金
中央高校基本科研业务费项目(#DL13EA01
#2572014CA03)
内蒙古贺兰山国家级自然保护区第一次综合科学考察项目
关键词
东方田鼠
传统形态分类学
DNA条形码技术
贺兰山
Microtus fortis
traditional morphological taxonomy
DNA barcoding technique
Helan Mountains
