摘要
针对油藏高温条件下微生物数量较少,驱油机理不够明确的特点,对胜利油田不同区块微生物进行筛选.得到一株能够乳化分散原油的细菌,经鉴定为嗜热脂肪地芽孢杆菌,能够耐受60℃以上的高温并能有效生长代谢,代谢产物主要由糖类、脂类和蛋白质组成.利用该菌的培养液、培养后代谢产物及培养后的菌体分别进行驱油实验,结果表明3种不同的驱替液均能够提高驱替效率,其中培养液能够提高驱替效率11.8%,其他两种驱替液提高驱替效率分别为4.5%和3%.培养液和培养后菌体驱替时岩心压力升高0.5 MPa以上,而培养后代谢产物驱替过程中压力变化不明显,但是代谢产物能够乳化剥离原油,同样达到提高驱替效率的作用.岩心驱替结束后对岩心内剩余油测定表明,从入口到出口呈现逐渐升高的趋势,证明微生物驱替时不断"推移"、"驱赶"原油的过程.综合实验结果,该菌株具备进一步现场实际微生物驱油应用的潜力.
An effective crude oil emulsion microbe SL-1 was isolated from Sheng Li oilfield. The microbe was identified as Geobacillus stearothermophilus by the molecular biology method. SL-1 could maintain normal growth and metabolism at 60 ℃ reservoirs and produce a metabolite composed of polysaccharide, fatty acids and proteins. The oil displacement experiments were carried out by using the culture liquid, metabolites and the bacteria respectively. The results showed that all of the displacement fluids could improve the displacement efficiency, which was enhanced by 11.8 by the culture fluid, 4.5% and 3% by the other two displacement fluids. The core pressure increased by 0.5 MPa for using culture medium and bacteria as displacement fluid, but the pressure change in the process of the metabolites was not obvious. The improvement of displacement efficiency by metabolic products was regarded to be through stripping of oil film. The determination of remaining oil in the core after flooding indicated that the residual oil gradually increased from the entrance to the exit, showing the process of SL-1 and its metabolites keeping driving out the crude oil in the core. The results indicated that SL-1 has potential applications in oil recovery.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2015年第6期1060-1064,共5页
Chinese Journal of Applied and Environmental Biology
基金
国家高技术研究发展计划(863计划
2013AA064401)资助~~
关键词
微生物驱油
嗜热脂肪地芽孢杆菌
乳化
驱油机理
驱替实验
MEOR
Geobacillus stearothermophilus
emulsification
oil displacement mechanism
displacement experiment
