摘要
为调查新疆北疆部分地区规模化奶牛场致犊牛腹泻冠状病毒的感染情况,查明该病毒在致犊牛腹泻中的作用及分子流行病学特征,从新疆北疆的石河子、沙湾、奎屯、呼图壁地区的主要奶牛场采集1月龄以内犊牛粪便119份,其中腹泻犊牛粪便71份,相同日龄无腹泻的健康犊牛的粪便48份。采用反转录聚合酶链反应技术直接从犊牛粪便中扩增出牛冠状病毒N基因（597bp）、S1基因（2 861bp）,并对牛冠状病毒S1基因进行测序、对比、分析。结果显示,从腹泻犊牛粪便中检出阳性样品12份,各牛场腹泻犊牛粪便的冠状病毒阳性检出率为0~38.89%,并未在健康犊牛粪便中检出牛冠状病毒。S1基因序列分析结果显示,与牛冠状病毒参考株Mebus、LY-138、OK、F15、L9、LSU的核苷酸和氨基酸序列的同源性分别在95.0%~97.7%、96.0%~98.6%之间。结果表明,此次调查的规模化奶牛场腹泻犊牛存在牛冠状病毒感染,所感染牛冠状病毒毒株与世界其他地区的参考毒株存在变异。
To investigate large-scale dairy calf diarrhea infection caused by coronavirus in parts of the northern region of Xinjiang,and to find out the role of this virus in calf diarrhea and molecular epidemiological characteristics,119 calf fecal samples at same age and less than one-month-old calves were collected from main dairy farm in Shihezi,Shawa,Kuitun and Hutubi of northern region of Xinjiang,among them,there were 71 diarrhoeic calf fecal samples and 48 healthy calf fecal samples.Reverse transcription polymerase chain reaction（RTPCR）method was used,and bovine coronavirus N gene（597bp）and S1gene（2 861bp）were amplified from feces of calf directly.Then bovine coronavirus S1 gene would be sequenced and analyzed.The results showed that 12 positive samples were detected with rates from 0to 38.89%in each cow dairy farm,bovine coronavirus was not detected from fecal samples of healthy calf.The sequencing result for S1 gene showed that the homology rate were from95.0%to 97.7%and from 96.0%to 98.6% with reference strains Mebus,LY-138,OK,F15,L9 and LSU on nucleotide sequence and amino acid sequence.This result suggested that calf diarrhea coronavirus infection was discovered in large-scale dairy of this investigation,variation was existed with strains of other areas of world.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2015年第12期1270-1276,共7页
Chinese Veterinary Science
基金
国家科技支撑计划项目(2012BAD43B02)
