摘要
目的：观察重型颅脑外伤（sever traumatic brain injury,s TBI）术后发生颅内感染的患者高迁移率族蛋白-1（High mobility group box 1,HMGB-1）和晚期糖基化终产物受体（receptor for advanced glycosylation end products,RAGE）的脑脊液（cerebrospinal fluid,CSF）水平,探讨其对诊断颅内感染的价值。方法：54例s TBI术后拟诊发生颅内感染的患者根据最后诊断分为颅内感染组（n=12）和非颅内感染组（n=42）。酶联免疫法吸附法（enzyme-linked immunosorbent assay,ELISA）检测拟诊颅内感染时CSF中的HMGB-1和RAGE蛋白水平。受试者工作曲线（receiver operating characteristic,ROC）分析其对颅内感染的诊断意义。结果：脑脊液的HMGB-1和RAGE水平在颅内染组和非颅内感染组之间比较差异具有统计学意义（t=6.711,P=0.000;t=2.683,P=0.008）。脑脊液HMGB-1和RAGE水平诊断颅内感染的ROC的曲线下面积分别为：0.899（95%CI：0.822~0.975）、0.682（95%CI：0.555~0.809）。HMGB-1的cut-off值分别为209.50 ng/m L（敏感度：0.75,特异性：0.93）;RAGE的cut-off值为108.50 ng/m L（敏感度：1.00,特异性：0.43）。结论：联合检测脑脊液HMGB-1和RAGE水平对s TBI术后是否发生颅内感染具有重要的诊断价值。
Objective： Observe the changes of cerebrospinal fluid（ CSF） levels of High mobility group box 1（ HMGB-1） and receptor for advanced glycosylation end products（ RAGE） in post-craniotomy of sever traumatic brain injury（ s TBI） patients with intracranial infection. Methods： The CSF concentrations of HMGB-1 and RAGE in post-craniotomy of s TBI patients with intracranial infection（ n = 12） and non-intracranial infection（ n = 42） were measured by a quantitative enzyme-linked immunosorbent assay（ ELISA）. Receiver operating characteristic（ ROC） curve verified the diagnostic value of HMGB-1 and RAGE protein levels in CSF.Results： CSF levels of HMGB-1 and RAGE in intracranial infection group were significantly higher than non-intracranial infection group（ t = 6. 711,P = 0. 000; t = 2. 683,P = 0. 008）. Area under the curve（ AUC） of HMGB-1 and RAGE ROC curve were 0. 899（ 95%CI： 0. 822 - 0. 975）,0. 682（ 95% CI： 0. 555 ~ 0. 809）,respectively. The best cut-off value of HMBG-1 for a diagnosis of intracranial infection was 209. 50 ng / m L（ sensitivity and specificity were 0. 75 and 0. 93）,the best cut-off value of HMBG-1 was 108. 50 ng / m L（ sensitivity and specificity were 1. 00 and 0. 43）. Conclusion： The CSF levels of HMGB-1 and RAGE were found to suitable biomarkers for diagnosing intracranial infection.
出处
《川北医学院学报》
CAS
2015年第6期748-751,共4页
Journal of North Sichuan Medical College
基金
郴州市第一人民院科研项目(N2010-055)
关键词
重型颅脑外伤
颅内感染
高迁移率族蛋白-1
晚期糖基化终产物受体
受试者工作曲线
Sever traumatic brain injury
Intracranial infection
High mobility group box-1
Receptor for advanced glycosylation end products
Receiver operating characteristic curve
