摘要
背景:哮喘的发病率逐年增高,建立和完善可以反映这种疾病的动物模型对于疾病研究具有重大意义。目的:探讨Balb/c与DO11.10小鼠哮喘模型的诱导方法,比较两者的差异。方法:24只Balb/c小鼠按随机数字表法等分为A-D组;用同样的方法把24只DO11.10小鼠等分为E-H组。A和E组小鼠第1天以PBS致敏,第8-11天以PBS雾化;B和F组小鼠第1天以卵清蛋白致敏,第8-11天以卵清蛋白雾化;C、G组小鼠第1,7,14天以PBS致敏,第22-25天以PBS雾化;D和H组小鼠第1,7,14天以卵清蛋白致敏,第22-25天以卵清蛋白雾化。结果与结论:1次致敏加上4次雾化只能引起Balb/c小鼠轻度的炎症反应,在经历3次致敏和4次雾化后Balb/c小鼠出现了气道反应性增高,肺部炎症细胞浸润,支气管杯状上皮黏液分泌增加,肺泡灌洗液炎症细胞和嗜酸性粒细胞数量显著上升,同时肺泡灌洗液中Th2型细胞因子水平也升高。DO11.10小鼠经过1次致敏和4次雾化即出现了显著的炎症反应,值得注意的是,其肺泡灌洗液中性粒细胞的数量也显著上升;而在经过3次致敏和4次雾化后,DO11.10小鼠炎症反应反而明显减轻。提示卵清蛋白可成功诱导出Balb/c和DO11.10小鼠的哮喘模型,不过两者在诱导方案和病理表现上有所差异。
BACKGROUND： The incidence of asthma increases year by year. Therefore, establishment and improvement of optimized animal models of asthma are important for the investigation of asthma.OBJECTIVE： To induce asthma method in Balb/c and DO11.10 mice and to compare their difference. METHODS： A total of 24 Balb/c mice were equally and randomly divided into groups A-D and 24 DO11.10 mice were equally divided into groups E-H. Mice in the groups A and E were induced to sensitization using PBS on day 1, and atomization using PBS on days 8-11. Mice in the groups B and F were induced to sensitization using ovalbumin on day 1, and atomization using ovalbumin on days 8-11. Mice in the groups C and G were induced to sensitization using PBS on days 1, 7 and 14, and atomization by PBS on days 22-25. Mice in the groups D and H were induced to sensitization using ovalbumin on days 1, 7 and 14, and atomization using ovalbumin on days 22-25.RESULTS AND CONCLUSION： One time of sensitization and four times of atomization only caused mild inflammatory reaction in the lungs in Balb/c mice. Three times of sensitization and 4 times of atomization caused clear airway hyperreactivity, inflammatory cell infiltration in lung, increased mucus secretion in bronchial goblet epithelium in Balb/c mice. Simultaneously, the number of inflammatory cells and eosinophils significantly increased in bronchoalveolar lavage fluid. Th2 type cytokine levels also increased in the bronchoalveolar lavage fluid. In DO11.10 mice, one time of sensitization and four times of atomization induced significant inflammatory response. Specially, the number of neutrophils obviously increased in the bronchoalveolar lavage fluid. However, after three times of sensitization and four times of atomization, inflammatory reaction significantly decreased in DO11.10 mice. These findings indicate that asthma model could be successfully induced with ovalbum in both Balb/c and DO11.10 mice. There were some differences in the induction and pathological features for asthma model
出处
《中国组织工程研究》
CAS
北大核心
2015年第49期7914-7919,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(81170896)
广东省科技计划(2012B031800045)~~
