摘要
背景:BIOSSN4无镍不锈钢是一种奥氏体医用不锈钢材料,在中国药品生物制品检定所通过了标准的溶血实验、细胞毒性实验和致敏实验。目的:评价新型医用BIOSSN4无镍不锈钢的体外细胞毒性及抗腐蚀性。方法:将小鼠L929成纤维细胞悬液以1×108 L-1的浓度接种于96孔板,分5组培养,分别加入BIOSSN4无镍不锈钢浸提液、316L不锈钢浸提液、金合金浸提液、铅材料浸提液(阳性对照)及RPMI1640培养液(阴性对照)。培养1,2,3 d,观察细胞形态,采用MTT法检测各组吸光度值,计算细胞相对增殖率,评价毒性分级。在模拟口腔环境中,检测BIOSSN4无镍不锈钢、316L不锈钢及金合金的自腐蚀电位、自腐蚀电流密度及极化电阻。结果与结论:培养3 d内,铅材料浸提液组细胞皱缩,细胞数量明显减少,细胞相对增殖率低于其余4组(P<0.05);其余4组细胞形态良好,增殖旺盛,细胞相对增殖率均在75%以上。BIOSSN4无镍不锈钢浸提液、316L不锈钢浸提液与金合金浸提液的毒性均为1级,铅材料浸提液的毒性为2至3级,表明BIOSSN4无镍不锈钢具有良好的生物相容性。BIOSSN4无镍不锈钢的抗腐蚀性高于316L不锈钢,低于金合金。
BACKGROUND：BIOSSN4 nickel-free stainless steel is an austenitic medical stainless steel material, which has passed the standard hemolysis test, cytotoxicity assays and sensitization test of the National Institute for the Control of Pharmaceutical and Biological Products. OBJECTIVE：To evaluate theinvitro cytotoxicity and corrosion resistance of a new medical BIOSSN4 nickel-free stainless steel. METHODS：The L929 mouse fibroblasts suspension was seeded in 96-wel plates at a concentration of 1×108 /L, and were divided into five groups. BIOSSN4 nickel-free stainless steel extract, 316L stainless steel extract, gold aloy extract, lead material extract （positive control） and RPMI1640 medium （negative control） were added respectively. After 1, 2 and 3 days of culture, cel morphology was observed. The absorbance value in each group was determined using MTT assay. The relative cel proliferation rate was calculated. Toxicity grading was evaluated. In the simulated oral environment, the eletric potential of corrosion, current density of corrosion and polarization resistance of BIOSSN4 no-nickel stainless steel, 316L stainless steel and gold aloy were determined. RESULTS AND CONCLUSION：Within 3 days of culture, in lead material extract group, cels shrunk; the number of cels significantly reduced; the relative growth rate was lower than that in the other four groups （P 〈 0.05）. In the other four groups, the cel morphology was good, and the relative growth rate was over 75%. The toxicity of BIOSSN4 nickel-free stainless steel extract, 316L stainless steel extract and gold aloy extract was grade 1. The toxicity of lead material extract was grades 2-3. These results demonstrate that BIOSSN4 nickel-free＆nbsp;stainless steel has good biocompatibility. The corrosion resistance of BIOSSN4 nickel-free stainless steel is higher than that of the 316L stainless steel but lower than that of the gold aloys.
出处
《中国组织工程研究》
CAS
北大核心
2015年第47期7608-7612,共5页
Chinese Journal of Tissue Engineering Research
基金
辽宁省博士启动基金(20141176)~~
