摘要
以NH_4HCO_3为造孔剂,采用粉末冶金烧结法制备低弹性模量、高孔隙度、孔隙尺寸适中和生物相容性良好的多孔CoTi合金。借助X射线衍射仪、金相显微镜、扫描电镜和力学实验机等对多孔CoTi合金的物相组成、孔隙特征和力学性能进行检测与分析。通过扫描电镜观测MG-63细胞的黏附情况及动物体内植入实验,采用Alamar blue法测定细胞增殖率。结果表明,多孔CoTi合金的孔隙特征受造孔剂特性和烧结温度的影响,细胞正常黏附并增殖。其中烧结温度为1 000℃,添加21%的NH_4HCO_3造孔剂,可制备出性能优异的多孔CoTi合金,该合金孔隙度为53.97%,平均孔隙尺寸为122μm,抗压强度44.8 MPa,弹性模量为2.16 GPa,这些孔隙特征和力学性能与人体松质骨相匹配,在培养6 h后MG-63细胞黏附形态完整,7d后增殖近一倍,植入动物体内12周后,材料与组织嵌合紧密,具有作为骨科替代材料的潜能。
CoTi alloys with low elastic modulus, high porosity, moderate pore size and good biocompatibility were prepared using NH4HCO3 as pore-fonning agent through powder metallurgy. The phase composition, microstructure and mechanical properties of porous CoTi alloys were investigated by X-ray diffraction, metallographic microscope, scanning electron microscope and mechanical testing instrument. Adhesion of MG-63 cells and vivo experiments on animals were observed by scanning electron microscope, and its proliferate rate was evaluated by Alamar blue method. The results show that the pore characteristics can be affected by the feature of the pore-forming agents as well as the sintering temperature, MG-63 cells can adhesion and proliferation properly; Porous CoTi alloy sintered at 1 000 ℃ with 21% addition of NHaHCO3 pore-forming agent exhibits the best performance, with the porosity, average pore size, compressive strength and elastic modulus being 53.97%, 122 lain, 44.8 MPa and 2.16 GPa respectively. The pore characteristics and mechanical properties coincide with the human cancellous bone, the MG-63 cells adhered on the surface for 6 h have perfect form and structure, and the number of cell is nearly doubled after 7 days. The materials and organization are tightly bound after implanted into the animals for 12 weeks, which indicates that porous CoTi alloys have the potential as bone substitute materials.
出处
《粉末冶金材料科学与工程》
EI
北大核心
2015年第6期873-880,共8页
Materials Science and Engineering of Powder Metallurgy
基金
中南大学中央高校基本科研业务费专项资金资助(2012zzts012)
湖南省博士生科研创新项目(CX2013D055)
国家自然科学基金资助项目(No.51274247)
国家高技术研究发展计划(863计划)资助项目(2012BAE06B00)
关键词
多孔CoTi合金
孔隙特征
力学性能
增殖率
porous CoTi alloy
pore feature
mechanical properties
proliferation rate
