摘要
目的:建立同时测定民族药芒萁中芦丁、槲皮苷、山柰酚-3-O-α-L-鼠李糖苷、槲皮素和山柰酚的RP-HPLC测定方法。方法:采用PntulipsTMBP-C18色谱柱(4.6 mm×250 mm,5μm),以乙腈(A)-0.5%磷酸(B)为流动相,梯度洗脱(0~10 min,15%A;10~11 min,15%A→25%A;11~20 min,25%A→55%A;20~30 min,55%A→15%A),流速1 m L·min^(-1),检测波长360 nm,柱温30℃。结果:在选定的实验条件下,芦丁、槲皮苷、山柰酚-3-O-α-L-鼠李糖苷、槲皮素和山柰酚得到良好的分离,质量浓度分别在77.2~694.8μg(r=0.999 8,n=6)、43.6~392.4μg(r=0.999 9,n=6)、288.0~2 592.0μg(r=0.999 9,n=6)、38.4~354.6μg(r=0.999 9,n=6)和43.8~394.2μg(r=0.999 2,n=6)范围内呈良好线性关系,平均回收率分别为97.6%(RSD=1.94%)、98.6%(RSD=1.11%)、96.9%(RSD=1.22%)、97.1%(RSD=1.97%)和95.3%(RSD=2.01%)。4个不同产地芒萁的含量测定结果表明,山柰酚-3-O-α-L-鼠李糖苷为芒萁的主要成分,其次为槲皮苷。不同产地的芒萁中5种成分的含量差异变化不大,芦丁、槲皮苷、山柰酚-3-O-α-L-鼠李糖苷、槲皮素和山柰酚的含量分别为0.109~0.176、0.584~0.686、2.416~2.627、0.088~0.131和0.018~0.031 mg·g^(-1)。结论:该方法简单易行,高效快速,可用于民族药芒萁的品质评价及质量控制。
Objective： To establish an HPLC-DAD method for simultaneous determination of the content of five main active ingredients-rutin,quercitrin,kaempferol-3-O-α-L-rhamnoside,quercetin and kaempferol in the medicine plant of Dicranopteris pedata. Methods： The HPLC separation was performed on a PntulipsTMBP-C18column（ 4. 6 mm × 250 mm,5 μm）,using acetonitrile（ A）-0. 5% phosphoric acid solution（ B） as the mobile phase in a gradient mode（ 0-（-1）0 min,15% A; 10-（-1）1 min,15% A →25% A; 11-20 min,25% A →55% A; 20-30 min,55% A→15% A） at a flow rate of 1 m L·min-（-1）. The detection wavelength was 360 nm,and the column temperature was 30 ℃. Results： A good separation of five compounds was achieved under the selected experimental conditions,showing a good linearity relationship separately within the range of 77. 2-694. 8 μg（ r = 0. 999 8,n = 6） for rutin,43. 6-392. 4 μg（ r = 0. 999 9,n = 6） for quercitrin,288. 0-2 592. 0 μg（ r = 0. 999 9,n = 6） for kaempferol-3-O-α-Lrhamnosides,38. 4-354. 6 μg（ r = 0. 999 9,n = 6） for quercetin and 43. 8-394. 2 μg（ r = 0. 999 2,n = 6） for kaempferol. The respective average recoveries were 97. 6%（ RSD = 1. 94%）,98. 6%（ RSD = 1. 11%）,96. 9%（ RSD = 1. 22%）,97. 1%（ RSD = 1. 97%）,and 95. 3%（ RSD = 2. 01%）. Determination results of Dicranopteris pedata from four different producing areas showed that kaempferol-3-O-α-L-rhamnoside was the main component of Dicranopteris pedata,followed by quercetin. The content of five kinds of compositions was similar to Dicranopteris pedata from different producing area. The contents of rutin,quercitrin,kaempferol-3-O-a-L-rhamnoside,quercetin and kaempferol ranged in 0. 109-0. 176,0. 584-0. 686,2. 416-2. 627,0. 088-0. 131 and 0. 018-0. 031 mg·g-（-1）,respectively. Conclusion： The established method is easy and efficient,and can be applied to determine the contents of five main active ingredients of the national drug Dicranopteris pedata.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2015年第12期2111-2115,共5页
Chinese Journal of Pharmaceutical Analysis
基金
贵州省科技创新人才团队建设项目[2013-4006]
关键词
芒萁
芦丁
槲皮苷
山柰酚-鼠李糖苷
槲皮素
山柰酚
高效液相色谱
民族药含量测定
Dicranopteris pedata
rutin
quercitrin
kaempferol-rhamnosides
quercetin
kaempferol
high performance liquid chromatography
ethnodrug quantitative analysis
