摘要
[目的]观察注射给予孕期持续摄取酒精母鼠外源性甲状腺素对新生子鼠大脑发育的影响.[方法]选择体质量及一般情况相似的SD孕鼠,用随机数字表法分为正常组、酒精组、酒精+T4组和代理母组.分娩6h后将酒精+T4组母鼠与子鼠分开,每天给予22%(体积分数)酒精,并颈后皮下注射给予5μg/kg甲状腺素.行麻醉后采集心脏血,测定血液中酒精及甲状腺素含量.新生子鼠由代理母组的母鼠养育,分别于出生后0,7,14,21,28 d(P0,P7,P14,P21,P28)采用免疫组织化学染色法观察子鼠大脑中BDNF和TrkB阳性神经细胞的形态及分布.[结果]酒精+T4组血液中酒精含量明显高于正常组(P<0.05),甲状腺素含量明显高于酒精组(P<0.05);P7时酒精+T4组大脑皮质中BDNF和TrkB阳性神经细胞形态及分布与正常组相似,可观察到长而明显突起的成熟的BDNF和TrkB阳性神经细胞,而酒精组始终未出现具有明显突起的成熟的BDNF和TrkB阳性神经细胞.[结论]注射给予孕期持续摄取酒精母鼠外源性甲状腺素可促进后代出生后早期大脑皮质中BDNF及其功能性受体TrkB的合成,促进BDNF和TrkB阳性神经细胞的发育,改善胎儿酒精效果所致低甲状腺素所引起的大脑发育障碍.
OBJECTIVE To observe the effects of exogenous thyroxine on cerebral cortex development of offspring in pregnant rats with continuous prenatal exposure to alcohol.METHODS SD rats with similar body mass and general condition were selected,and were divided into the control group,alcohol group,alcohol＋T4 group and surrogate mother group by random number table.After 6th days of delivery,the mother rats and offspring in the alcohol＋T4 group were separated to give the 22%（volume fraction）alcohol,and to inject with 5μg/kg thyroxine on posterior neck.Heart blood was taken after anesthesia,and the plasma concentrations of alcohol and thyroxine were detected.The newborn rats in surrogate mother group were fostered by surrogate mothers,and the morphology and distribution of neurons with containing the BDNF-and TrkB-in the cerebral cortex of offspring at 0,7,14,21 and 28 postnatal days（P〈0,P7,P14,P21,P28）were observed by immunohistochemistry.RESULTS The alcohol concentration in blood was significantly higher in alcohol＋T4group than in control group（P〈0.05）,and thyroxine concentration was significantly higher in alcohol＋T4group than in alcohol group（P〈0.05）.The morphology and distribution of cortical neurons with containing the BDNF-and TrkB-in alcohol＋T4group at P7 were similar to those in control,and it was observed that long,obviously protuberant and matured cortical neurons with containing the BDNF-and TrkB-,and in contrast,there were no matured cortical neurons with containing the BDNFand TrkB-in alcohol group during postnatal life all the time.CONCLUSIONExogenous thyroxine injected in vivo to pregnant rats with continuous prenatal exposure to alcohol could promote the synthesis of the BDNFand TrkB-in early cerebral cortex of offspring during postnatal life and development of cortical neurons with containing the BDNF-and TrkB-,and improve the brain developmental disorder of rat＇s fetus induced by low thyroxine resulted from the alcohol.
出处
《延边大学医学学报》
CAS
2015年第3期164-167,共4页
Journal of Medical Science Yanbian University
