摘要
目的研究白塞病(Behcet’s disease,BD)患者外周血CD4^+T细胞和CD8^+T细胞上ICOS(inducible costimulatory molecule,ICOS)与CD28共表达水平及与疾病的关系。方法采用流式细胞术检测BD患者(n=22),包括BD活动期(active BD,ABD)患者(n=14)和BD稳定期(stable BD,SBD)患者(n=8),以及健康体检者(n=22)外周血CD4^+T细胞及CD8^+T细胞上ICOS与CD28共表达水平。结果 ABD患者外周血CD28^+ICOS^+CD4^+T细胞的比例较正常对照明显上升[(34.72±12.87)%vs.(25.36±8.24)%,P<0.05],CD28^+ICOS^+CD8^+T细胞的比例与正常对照相比无明显改变[(9.32±4.57)%vs.(7.24±3.36)%,P>0.05];ABD患者外周血CD28^-ICOS^+CD4^+T细胞的比例较正常对照明显升高[(2.54±1.63)%vs.(0.76±0.25)%,P<0.05];ABD患者CD28^-ICOS^+CD8^+T细胞的比例较正常对照也明显升高[(8.79±3.41)%vs.(3.04±1.25)%,P<0.05]。结论 ICOS在BD患者外周血T细胞亚群中表达水平的增高在一定程度上独立于CD28分子,提示患者体内仅表达ICOS分子的细胞同样也可能在发病过程中发挥重要作用,为BD的治疗提供了相应的理论依据。
Objective To study the relationship between the co^-expression levels of inducible costimulatory molecule（ICOS）and CD28 on peripheral blood CD4~＋and CD8~＋T cells and Behcet＇s disease（BD）.Methods Flow cytometry was used to detect the expression levels of ICOS and CD28 on peripheral blood CD4~＋and CD8~＋T cells of BD patients（n=22）,including those with active BD（ABD,n=14）and stable BD（SBD,n=8）,and of normal subjects（n=22）.Results The proportion of CD28~＋ICOS~＋CD4~＋T cells was significantly increased in ABD patients when compared with normal subjects[（34.72±12.87）% vs.（25.36±8.24）%,P〉0.05].No significant difference was found in the proportion of CD28~＋ICOS~＋CD8~＋T cells[（9.32±4.57）% vs.（7.24±3.36）%,P〈0.05]between the two groups.The proportions of CD28^-ICOS~＋CD4~＋T cells and CD28^-ICOS~＋CD8~＋T cells were both obviously increased in ABD patients when compared with those in normal subjects[（2.54±1.63）% vs.（0.76±0.25）% for CD28^-ICOS~＋CD4~＋T cells,P〈0.05;（8.79±3.41）% vs.（3.04±1.25）% for CD28^-ICOS~＋CD8~＋T cells,P〈0.05].Conclusion The increased expression level of ICOS on peripheral blood CD4~＋and CD8~＋T cells is not closely associated with the expression of CD28 to some extent in BD patients,suggesting that cells expressing only ICOS play an important role in the development of BD.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2015年第6期718-721,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
关键词
白塞病
可诱导共刺激分子
细胞表面分子
Behcet＇s disease
inducible costimulatory molecule（ICOS）
cell surface molecule
