摘要
目的制备一种能提高T细胞对癌细胞杀伤能力的双特异性抗体。方法利用生物可降解材料PLGA(聚乳酸-羟基乙酸共聚物)制备成纳米颗粒,然后利用化学方法在纳米颗粒表面偶联能靶向结合T细胞的CD3抗体和癌细胞标志物MUC1的抗体,利用T细胞对癌细胞的杀伤实验来检测其功能。结果成功制备出直径200 nm的PLGA纳米颗粒并偶联上CD3和MUC1抗体,功能检测得出制备的双特异性抗体能显著提高T细胞对癌细胞的杀伤率。结论制备出一种能显著提高T细胞对癌细胞杀伤率的双特异性抗体。
The aim of this study is to prepare a kind of bispecific antibody that can enhance the ability of T cells against tumor cells. Nanoparticles were made with biodegradable material PLGA（polylactic acid- glycolic acid）, then CD3 and MUC1 antibodies were crosslinked to the surface of nanoparticles to make a kind of bispecific antibody with chemical methods, and the function of the bispecific antibody was tested by CCK-8 assay. Data showed that the diameter of the PLGA nanoparticle bispecific antibody was about 200 nm, and the CD3-MUC1 bispecific antibody obviously improved the kill rate of T cells to MCF-7, A549 and MD-MBA-231 cells in vitro. In conclusion,a kind of bispecific antibody that can significantly enhance the ability of T cells against tumor cells is successfully prepared.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2016年第1期34-37,共4页
Immunological Journal
基金
深圳市海外高层次人才创新创业专项资金(KQCX20120806161031208)
