摘要
为探索杜仲胚性愈伤组织诱导的条件,建立杜仲体细胞胚胎发生初步体系,以杜仲幼嫩叶片和未成熟合子胚为外植体、MS为基本培养基,探究外源激素配比、未成熟合子胚发育阶段与基因型对愈伤组织诱导的影响,并从形态学和细胞学对愈伤组织进行胚性的判断。试验结果表明:4种不同激素配比的培养基诱导出的叶片愈伤组织在形态上具有差异,MS+2,4-D 2 mg/L+6-BA 1 mg/L和MS+2,4-D2 mg/L+6-BA 0.5 mg/L有利于叶片胚性愈伤组织诱导;在培养基MS+2,4-D 2 mg/L+6-BA 1 mg/L上,以未成熟合子胚为外植体诱导出4种类型愈伤组织,其中圆球形和颗粒型突起的愈伤组织具有胚性;未成熟合子胚采集时间对愈伤组织诱导率具有显著差异,6月14日采集的外植体愈伤诱导率最高,不同基因型差异不显著。
In order to explore the embryonic callus conditions and establish a preliminary system of somaticembryogenesis of Eucommia ulmoides, the influence of different hormone proportions in medium and immatureembryo age and genotype on the induction of embryogenic callus of Eucommia ulmoides was explored usingyoung leaves, immature zygotic embryos as explants. In addition, embryogenic callus was distinguished via themorphology and cytology. The results showed that the leaf callus induced from 4 kinds of different hormoneproportion mediums were different in color and texture, in the medium of MS＋ 2,4-D 2 mg/L＋ 6-BA 1 mg/Land MS ＋ 2,4- D 2 mg/L ＋ 6- BA 0.5 mg/L, embryogenic callus of leaves was easier to be induced. In themedium of MS＋ 2,4-D 2 mg/L＋ 6-BA 1 mg/L, the callus induced from the immature zygotic embryos could bedivided into four categories： the types of spherical and granular callus were embryogenic. The immature zygoticembryos acquisited from different periods had significant differences in callus induction rates and June 14 th showed the highest callus induction rate, while callus induction of different genotypes had no significantdifference.
出处
《中国农学通报》
2015年第34期20-27,共8页
Chinese Agricultural Science Bulletin
基金
北京林业大学大学生创新创业训练项目"杜仲胚性愈伤组织的诱导及增殖研究"(X201410022033)
国家自然科学基金项目"科研训练及科研能力提高项目"(J1103516)
国家林业局林业公益性行业专项"自然保护区成效监测
评估与预警关键技术研究"(201404422)
关键词
杜仲
叶片
未成熟胚
愈伤组织
胚性愈伤组织
Eucommia ulmoides Oliv.
leaf
immature embryo
callus
embryogenic callus
