摘要
目的 :观察戈登链球菌(S.gordonii)对牙龈卟啉单胞菌(P.gingivalis)生物膜超微结构及生物膜中牙龈卟啉单胞菌数量的影响。方法:分别形成P.gingivalis单菌种生物膜和P.gingivalis-S.gordonii混合生物膜,利用扫描电镜观察其超微结构,定量PCR法对生物膜中P.gingivalis数量进行定量分析。采用SPSS 13.0软件包对数据进行统计学分析。结果:P.gingivalis生长72 h,与单菌种生物膜相比,P.gingivalis-S.gordonii混合生物膜形成量明显增多;而且混合生物膜的超微结构更规则、有序、多孔隙。在生物膜形成24、48、72 h后,混合生物膜中P.gingivalis的数量分别是单菌种生物膜中P.gingivalis数量的5.4、3.8和4.4倍。结论:早期定植菌S.gordonii的存在,使P.gingivalis更容易定植、形成生物膜。
PURPOSE： To investigate the impact of S.gordonii on the ultrastructure of P.gingivalis biofilm and on the amount of P.gingivalis in biofilm.METHODS： P.gingivalis and/or S.gordonii grew on the culture slides to form single P.gingivalis biofilm and heterotypic biofilm of P.gingivalis-S.gordonii.Then the ultrastructure of the 2 kinds of film were examined by scanning electron microscope,and the amount of P.gingivalis in the biofilm was detected by q PCR.Statistical analysis was performed using pair t test with SPSS 13.0 statistical package.RESULTS： At 72 h,the amount of heterotypic biofilm was much more than that of the single P.gingivalis biofilm.Moreover,the structure of the heterotypic biofilm was more regular and with more pore space compared to the single P.gingivalis biofilm.Compared to single P.gingivalis biofilm,the amount of P.gingivalis in heterotypic biofilm was 5.4,3.8 and 4.4 fold at 24,48 and 72 h,respectively.CONCLUSIONS： The growth of P.gingivalis was promoted by S.gordonii in the form of heterotypic biofilm compared to single P.gingivalis biofilm.
出处
《上海口腔医学》
CAS
CSCD
2015年第6期641-644,共4页
Shanghai Journal of Stomatology
基金
国家自然科学基金(81400518,81470745)
人力资源部和社会保障部留学人员项目
国家临床重点专科资助项目~~
