摘要
目的探讨大鼠间隙连接蛋白43(Cx43)和骶髓N-甲基-D-天冬氨酸(NMDA)受体与肠易激综合征(IBS)内脏敏化的关系。方法雄性SD大鼠50只,随机分为正常对照组、正常结肠扩张组、IBS组、IBS结肠扩张组及IBS结肠扩张+甲磺酸伊马替尼(STI-571)干预组,每组10只。IBS组、IBS结肠扩张组和IBS结肠扩张并干预组大鼠采用旋毛虫感染制作IBS模型。采用免疫荧光组织化学法观察各组大鼠结肠Cx43和骶髓NMDA受体的表达。结果正常组、正常结肠扩张组、IBS组的结肠Cx43和骶髓NMDA表达水平差异无统计学意义(P>0.05),IBS结肠扩张组结肠Cx43和骶髓NMDA表达水平与正常组、正常结肠扩张组、IBS组比较均显著增强(P<0.01),而STI-571干预后大鼠结肠Cx43和骶髓NMDA表达均有所下降(P<0.01)。结论 Cx43和NMDA可能在IBS内脏敏化机制中起关键作用。
Objective To study the relationship between connexin 43 （Cx43） and N-methyl-D-aspartate （NMDA） receptors and visceral sensitization in the rats with irritable bowel syndrome （IBS）. Methods Thirty rats were garaged with Triehinella spiralis to reproduce the IBS model. These rats were randomly divided into IBS group, IBS＋colon distension group, and IBS＋STI-571＋colon distension group, and other groups of normal rats were randomized into normal group and normal＋colon distension group, with 10 rats in each group. Immunofluorescent double staining were used to observe the expressions of intestine Cx43 and sacral NMDA receptors of rats in all the groups. Results The Cx43 and sacral NMDA expressions in the normal group, normal＋colon distension group and IBS group showed no significant changes （P〉0.05）, however, Cx43 and sacral NMDA expressions were significantly higher in IBS rats with colon distension as compared with those in normal group, normal＋colon distension group, and IBS group （P〈0.05）, while they were significantly lower in the IBS＋STI-571＋colon distension group after STI-571 intervention （P〈0.05）. Conclusion Cx43 and sacral NMDA may be the most important factor of visceral sensitization in IBS rats.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2015年第12期946-949,共4页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金(81070306)~~
