摘要
目的用免疫组化法及超微病理技术探讨烧伤湿性医疗技术(Moist Exposed Burn Therapy,MEBT)及其配套产品湿润烧伤膏(Moist Exposed Burn Ointment,MEBO)促进大鼠糖尿病足溃疡创面愈合的机制。方法将100只SPF级雄性SD大鼠随机分为空白对照组(20只)与糖尿病造模组(80只)。采用腹腔注射链脲佐菌素(STZ)制备大鼠糖尿病模型,选取60只造模成功的大鼠并随机分为MEBO组、贝复济组、模型组(每组20只)。4组大鼠均建立足溃疡模型,空白对照组、模型组外敷生理盐水,MEBO组外敷湿润烧伤膏,贝复济组外敷贝复济。治疗第5天和第14天,各组分别处死10只大鼠,利用电镜观察溃疡创面肉芽组织细胞的超微结构,并用免疫组化技术测定TGF-β1、Smad3蛋白的表达量,对比各组观察及测定结果。结果治疗第5天和第14天,MEBO组与贝复济组溃疡创面肉芽组织细胞的超微结构逐步改善,细胞内各细胞器的形态结构逐渐恢复,MEBO组、贝复济组肉芽组织细胞的超微结构情况明显优于模型组。连续换药第5天,MEBO组TGF-β1、Smad3蛋白的表达量均明显高于模型组(P<0.001),且MEBO组、空白对照组、贝复济组3组相比,TGF-β1、Smad3蛋白的表达量无明显差异;第14天,MEB0组TGF-β1、Smad3蛋白的表达量均明显低于空白对照组(P<0.001),且MEBO组、贝复济组、模型组3组相比,TGF-β1、Smad3蛋白的表达量无明显差异。结论 MEBT/MEBO能改善细胞的超微结构,并通过动态调节TGF-β1、Smad3蛋白的表达量,促进糖尿病足溃疡创面的愈合,同时可在一定程度上减少瘢痕形成。
Objective To explore the mechanism of Moist Exposed Burn Therapy（MEBT） and its supportive product Moist Exposed Burn Ointment（MEBO） in promoting diabetic foot ulcer wound healing in rats by using the immunohistochemical method and ultrastructural pathological techniques.Methods One hundred SPF male SD rats were randomly divided into a blank control group（20 rats） and a diabetes model group（80 rats）.Diabetic rat models were prepared in the diabetes model group by intraperitoneal injection of Streptozotocin（STZ） and finally 60 diabetic rats were selected to be randomly divided into MEBO group,the bovine basic fibroblast growth factors（bFGF） group and model group（20 rats in each）.After foot ulcer models were established in rats of all the four groups,the blank control group and the model group were dressed with normal saline,while MEBO group was dressed with MEBO and bFGF group was dressed with the bovine basic fibroblast growth factors.Five and fourteen days later,10 rats were put to death in each group respectively.Ultrastructure of granulation tissues of ulcer wounds was observed by using TEM microscope and the expression levels of Protein TGF-β1 Smad3 were detected using immunohistochemical techniques,of which the results were compared between groups.Results On the day 5 and 14 of treatment,the cellular ultra-structures of granulation tissues in MEBO group and bFGF group were gradually improved,with gradual recovery of organelles in terms of morphology and structure,which was obviously superior to that in the model group.After 5 days of continuous dressing,the expression levels of Protein TGF-β1 Smad3 in MEBO group were obviously higher compared with the model group（P〈0.001）,while the expression levels of Protein TGF-β1 Smad3 in MEBO group,blank control group and bFGF group didn＇ t differ significantly.On the day 14,the expressions of protein TGF-β1 Smad3 in MEBO group were both significantly lower than that in the blank control group（P〈0.001）,while there were no sig
出处
《中国烧伤创疡杂志》
2015年第6期392-405,共14页
The Chinese Journal of Burns Wounds & Surface Ulcers
基金
2013年广西自然科学基金重点项目(项目批准号:2013GXNSFDA019020)
2014年广西中医药科技专项课题(项目批准号:GZSY14-08)~~
