摘要
目的检测诱骗受体3（DcR3）在胰腺癌组织中的表达，探讨DcR3对胰腺癌患者诊断、治疗、预后判断的意义。方法应用免疫组织化学方法（Envision法）检测胰腺癌组织（n=100）、癌旁组织（n=15）及正常胰腺组织（n=15）中DcR3蛋白的表达水平。结果患者胰腺癌组织中DcR3蛋白阳性率比癌旁组织高（86．0％比46．6％，P〈0．05），癌旁组织中DcR3蛋白阳性率高于正常组织（46．6％比13．3％，P〈0．05）；Ⅲ期胰腺癌组织DcR3阳性率高于Ⅱ、Ⅰ期（100％比87．0％，P〈0．05；100％比62．5％，P〈0．05）；三期比较差异有统计学意义（P〈0．05）。有淋巴结转移组DcR3阳性率高于无淋巴结转移组（93．4％比79．6％，P〈0．05）；低分化胰腺癌组织中DcR3表达阳性率高于高分化组（100％比64．0％，P〈0．05）；中分化组DcR3表达阳性率高于高分化组（88．6％比64．0％，P〈0．05），三者相比P〈0．05。结论胰腺癌患者DcR3蛋白的表达水平从正常胰腺组织、胰腺癌癌旁组织到胰腺癌组织逐步升高，并与患者的临床分期、组织分化程度及淋巴结转移情况密切相关，与年龄、性别、胰腺癌肿瘤直径大小无关。
Objective To detect the expressions of decoy receptor 3 （ DcR3 ） in pancreatic cancer tissues and to analyze the significance of DeR3 in the diagnosis, treatment and prognosis of patients with pancreatic cancer. Methods The expressions of DcR3 in pancreatic cancer tissues （ n = 100） , paracancer tissues （ n = 15 ） and normal tissues （ n = 15 ） were detected with immunohistochemieal method （ Envision method）. Results The positive rate of DcR3 in pancreatic cancer tissues was significantly higher than that in adjacent-tumor pancreatic cancer tissues （86.0% vs. 46.6% , P 〈 0.05）. The positive rate of DcR3 in adjacent-tumor pancreatic cancer tissues was significantly higher than that in normal tissues （46. 6% vs. 13.3% , P 〈 0.05 ）. In clinical stage m, the positive rate of DcR3 was significantly higher than that in stage Ⅲ and stage Ⅰ（100% vs. 87.0%, P〈0.05; 100% vs. 62.5%, P〈0.05）. There were significant differences among the three groups （ P 〈 0.05 ）. With lymph node metastasis, the DcR3 positive rate was significantly higher than that in the group without lymph node metastasis （93.4% vs. 79.6% , P 〈 0. 05 ）. In poorly differentiated pancreatic cancer, the positive rate of DcR3 was significantly higher than that in the highly differentiated group （ 100% vs. 64.0%, P 〈0.05）, the positive rate of DcR3 was significant- ly higher in the moderately differentiated group than that in the highly differentiated group （ 88. 6% vs. 64.0% , P 〈 0.05 ）, There were significant differences among the three groups （ P 〈 0. 05 ）. There was no significant difference in the positive rate of DcR3 between the different age groups or the different gender groups （ P 〉 0.05）. Conclusions The expression levels of DcR3 in patients with pancreatic cancer gradually increased from normal tissues to paracancer tissues, to pancreas tissues. The expression level of DcR3 protein was closely related to clinical stage, degree of tissue differentiation and presence of lym
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2015年第12期830-832,共3页
Chinese Journal of Hepatobiliary Surgery
关键词
诱骗受体3
胰腺癌
免疫组化
蛋白表达
DcR3
Pancreatic cancer
Immunohistochemistry
Protein expression
