摘要
目的:探讨紫菀酮的体外抗炎作用及其机制分析。方法:利用脂多糖诱导RAW264.7巨噬细胞建立细胞炎症反应模型,通过紫菀酮对其进行干预,采用Sun BioTMAm-Blue细胞增殖与活性检测试剂检测紫菀酮对RAW264.7巨噬细胞增殖的影响;Griess法检测细胞培养上清液中NO含量;ELISA法检测细胞培养上清液中白细胞介素^(-1)β(IL^(-1)β)和肿瘤坏死因子-α(TNF-α)含量。结果:与空白组比较,50μmol·L^(-1)紫菀酮组RAW264.7巨噬细胞的增殖抑制率明显高于空白组,0.1,1,10,20,30μmol·L^(-1)紫菀酮组RAW264.7巨噬细胞的增殖抑制率无显著性差异;10,30μmol·L^(-1)紫菀酮组NO释放量均显著低于脂多糖组;1,10,30μmol·L^(-1)紫菀酮组IL^(-1)β和TNF-α释放量均显著低于脂多糖组。结论:紫菀酮呈剂量依赖性抑制脂多糖诱导的RAW264.7巨噬细胞炎性细胞因子IL^(-1)β,TNF-α及NO的释放,具有体外抗炎作用。
Objective： To explore anti-inflammatory mechanism in vitro of Shionone. Method： Cellular inflammatory reaction model of RAW264. 7 macrophages was induced by lipopolysaccharide, this model was intervened by shionone,effect of shionone on proliferation of RAW264. 7 macrophages was detected by Sun BioTM Am-Blue cell proliferation and active detection reagent. The content of NO in cell culture supernatants was assessed by Griess reaction,levels of tumor necrosis factor-α（ TNF-α） and interleukin-1β（ IL-1β） were measured by commercially available ELISA kit. Result： Compared with the blank group,proliferation inhibition rate of RAW264. 7 macrophages in the 50 μmol·L-1shionone group significantly increased,but this parameter in other shionone groups had no obvious change. Compared with the lipopolysaccharide group,releases of NO in 10,30μmol·L-1shionone group significantly decreased,levels of TNF-α,IL-β in 1,10,30 μmol·L-1shionone group significantly decreased. Conclusion： Inhibition of shionone on release of TNF-α, IL-1β and NO shows dosedependent. Shionone has anti-inflammatory activity in vitro.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第24期123-125,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江西省科技支撑项目(20111BBG70005-4)
江西中医药大学青年基金项目(Y027)
江西中医药大学重点学科青年教师培养项目(2015jzzdxk012)
