摘要
本试验旨在研究饲料胆碱含量对斜带石斑鱼生长性能、体成分、肝脏胆碱含量及脂肪代谢酶活性的影响,并确定斜带石斑鱼对饲料中胆碱的需要量。在基础饲料中分别添加0、680、1 361、2 041、2 721和4 082 mg/kg的氯化胆碱,配制6种试验饲料,试验饲料中胆碱实际含量分别为8.1(对照)、602.5、1 119.0、1 511.5、1 970.0和4 029.0 mg/kg,投喂360尾平均体重为(87.85±0.04)g的斜带石斑鱼10周。每种饲料设3个重复,每个重复放养20尾鱼。结果表明:1 119.0 mg/kg组的特定生长率、蛋白质效率最高,饲料系数最低;对照组的成活率显著低于其他各添加组(P<0.05)。饲料胆碱含量对全鱼水分和粗蛋白质含量无显著影响(P>0.05);4 029.0 mg/kg组的全鱼粗脂肪含量显著高于对照组(P<0.05);对照组的全鱼粗灰分含量与各胆碱添加组差异不显著(P>0.05)。肝脏胆碱含量随饲料胆碱含量的增加呈先升高后稳定趋势,且在1 511.5 mg/kg组有最大值。随饲料胆碱含量的增加,肝脏脂蛋白脂酶和总脂酶活性呈上升趋势,肝脏脂肪酸合成酶活性先下降后趋于稳定;肝脏肝脂酶活性不受饲料胆碱含量的显著影响(P>0.05)。以特定生长率和肝脏胆碱含量为评价指标进行折线模型分析得出,斜带石斑鱼对饲料中胆碱的需要量分别为1 093.7和1 579.7 mg/kg饲料。
A 10-week feeding trial was carried out to investigate the effects of dietary choline content on growth performance,body composition,and choline content and lipid metabolism enzyme activities in liver of orangespotted grouper（ Epinephelus coioides）. Choline chloride was added to the basal diet to formulate six experimental diets with the adding levels were 0,680,1 361,2 041,2 721 and 4 082 mg / kg,which contained 8. 1（ control）,602. 5,1 119. 0,1 511. 5,1 970. 0 and 4 029. 0 mg / kg（ all were measured values） choline,respectively. Each diet was randomly assigned to three replicates and each replicate had 20 fish [average body weight of（ 87. 85±0. 04） g]. Results showed that the maximum specific growth rate and protein efficiency rate and minimum feed conversion ratio were reached at 1 119. 0 mg / kg group. Survival rate in control group was significantly lower than that in other groups（ P〈0. 05）. No significant differences in moisture and crude protein content of whole-body were found among the groups（ P〉0. 05）, and ether extract content of whole-body in4 029. 0 mg / kg group was significantly higher than that in control group（ P〈0. 05）. No significant difference in ash content of whole-body was obversed between the control group and choline added groups（ P〉0. 05）. The choline content in liver was progressively increased and then leveled off with the increasing of dietary choline content and a maximum value was occurred in 1 511. 5 mg / kg group（ P〈0. 05）. Liver lipoprotein lipase and total lipase activities were tended to increase,but liver fatty acid synthetase activity was tend to decrease and then stable with the increasing of dietary choline content; no significant difference was observed in hepatic lipase activity in liver among all groups（ P〈0. 05）. Base on the specific growth rate and liver choline content as evaluation indexes,the choline requirement for orange-spotted grouper determined by broken-line model analysis is 1093. 7 and 1 579. 7 mg / kg diet
出处
《动物营养学报》
CAS
CSCD
北大核心
2015年第12期3812-3820,共9页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
公益性行业(农业)科研专项(201003020)
广东省高等学校珠江学者特聘教授基金(GDUPS2011)
广东省教育厅科技创新基金(2013KJCX0096)
关键词
斜带石斑鱼
胆碱
生长
体组成
需要量
脂肪代谢
orange-spotted grouper（Epinephelus coioides）
choline
growth
body composition
requirement
lipid metabolism
