摘要
目的观察及评价低聚糖对高强度训练时肠道屏障、氧化应激和炎症标志物的影响。方法64名集训武警战士接受40d的高强度训练，随机均分为低聚糖组和对照组，低聚糖组每人每日服用低聚糖制剂300mL。集训前1d和集训后24h取样，检测粪便中肠道屏障标志物，检测静脉血中氧化应激和炎症标志物。结果①集训后24h低聚糖组粪便中的人连蛋白浓度显著低于集训前和对照组（均P〈0．05），集训前后α1-抗胰蛋白酶浓度差异无统计学意义。②两组集训前蛋白质羟基化合物（CP）均稍高于正常值。集训后低聚糖组CP降至正常，与集训前和对照组相比均P〈0．05。集训40d后低聚糖组肿瘤坏死因子--α（TNF-α）含量显著低于集训前（P〈0．05）。③集训前后两组总氧化状态（TOS）、丙二醛（MDA）和白细胞介素-6（IL-6）的浓度差异无统计学意义（均P〉0．05）。结论低聚糖能够促进集训后的肠道屏障功能，降低持续训练造成的氧化应激和炎症反应。
Objective To invastigate the effects of oligosaccharides on the markers of intestinal barrier, oxidative stress, and inflammation in intensive training. Methods 64 armed police soldiers undergoing intensive training for 40 days were randomly divided into 2 equal groups： control group following their normal diet and oligosaceharide group receiving 300 mg of oligosaccharides every day in addition. One day before and 24h after the training stool samples were collected to measure the concentrations of zonulin and ot 1-antitrypsin to estimate the gut leakage. Venous blood samples were collected to determine the concentrations of carbonyl proteins （CP）, malondialdehyde （MDA）, total oxidation status of lipids （TOS）, tumor necrosis factor-alpha （TNF-α）, and interleukin-6 （IL-6）. Results （1） The concentration of zonulin in stool after training of the oligosaccharide group was significantly lower than that before training, and significantly lower than that after training of the control group as well （both P 〈0.05）. （2） The CP concentrations of these groups were both slightly high than normal before training. The CP concentration after training of the oligosaccharide group was significantly lower than that before training （P 〈0.05）, and significantly lower ＊than that after training of the control group as well （P 〈0.05）. The concentration of tumor necrosis factor-α （TNF-α） after training of the oligosaccaride group was significantly lower than that before trainiong （P 〈0.05）. （3） There were no significant differences in the concentrations of malondialdehyde, total oxidation status of lipids, and interleukin- 6 before and after training in both groupos （all P 〉0.05）. Conclusion Oligosaccharides helps improve the intestinal barrier function, and reduce the oxidative stress and inflammatory reaction caused by intensive training.
出处
《中国急救复苏与灾害医学杂志》
2015年第12期1143-1146,共4页
China Journal of Emergency Resuscitation and Disaster Medicine
关键词
低聚糖
集训武警战士
肠道屏障
氧化应激
炎症反应
Oligosaccharides
Armed police soldiers
Intestinal barrier
Oxidative stress
Inflammatio
