摘要
目的:观察异氟烷宫内暴露对胎鼠海马神经元凋亡的影响。方法:将妊娠19 d的SD大鼠随机分为异氟烷麻醉组(I组)和对照组(C组),I组孕鼠接受3 h的1.3%异氟烷吸入麻醉,C组吸入空气。采用原位末端标记法(TdT mediated dUTP nick end labeling,TUNEL)观察胎鼠大脑海马CA1区神经元在异氟烷暴露结束后0、3 h和9 h时间点的凋亡情况。结果:I组和C组海马CA1区神经元在异氟烷暴露后0、3 h和9 h均见凋亡;2组异氟烷暴露后0h和3 h凋亡指数差异均有统计学意义(P<0.05),暴露后9 h凋亡指数差异无统计学意义(P>0.05)。结论:1.3%异氟烷宫内暴露不会加重胎鼠海马CA1区神经元的凋亡,在暴露后早期反而有减缓作用,提示胎儿期可能不是异氟烷发育神经毒性的易损期。
Objective：To observe the effect of in-utero exposure to isoflurane on apoptosis of hippocampal neuron in fetal rats.Methods：SD rats at gestational day 19 were randomly assigned into the isoflurane anesthesia group（group I）and the control group（group C）.The rats in group I were exposed to 1.3% isoflurane for 3 h,while group C were exposed to air.The Apoptosis status of neurons in hippocampal CA1 region of fetal brains were observed by using TdT mediated dUTP nick end labeling（TUNEL）0h,3 h,and 9 h after exposure to isoflurane.Results：There were a certain amount of apoptotic neurons in hippocampal CA1 region in both group I and group C0 h,3 h,and 9 h after the exposure.There were significant differences regarding apoptosis index（AI）0h and 3 h after the exposure between the two groups（P〈0.05）.However,there was no significant difference regarding the AI between the two groups 9 h after the exposure（P〉0.05）.Conclusions：The in-utero exposure to 1.3%isoflurane does not increase the apoptosis of neurons in hippocampal CA1 region of fetal rats.Furthermore,it may alleviate the apoptosis during the early period.The result suggests that fetus period may not be the vulnerable period for isoflurane-induced neurotoxicity.
出处
《中国临床医学》
2015年第5期579-581,共3页
Chinese Journal of Clinical Medicine
基金
上海市科学技术委员会医学引导资助项目(编号:14411966700)
上海市卫生和计划生育委员会基金面上项目(编号:20124287)
关键词
异氟烷
宫内暴露
凋亡
海马神经元
Isoflurane
In-utero exposure
Apoptosis
Hippocampal neuron
