摘要
利用固态发酵法生产根霉脂肪酶,对影响此酶分离的多种条件进行了优化研究,其中包括浸提固液比、浸提时间、硫酸铵饱和度和分子排阻凝胶层析的洗脱流速。得出了优化后条件为浸提固液比为1∶3,浸提时间为120min,硫酸铵饱和度为40%,洗脱流速为1.5mL/min的结论。
By using solid state fermentation, the article aims to produce rhizopus lipases and researches conditions that affect this enzyme separation, whichincludethe solid--to--liquidof extraction, time saturation of ammonium sulfateand elution flow rate of molecular exclusion gel chromatography. After theconditionsare that the solid--to--liquid ratiois 1 ： 3, time of extraction is 120 minutes, saturation sulfate is 40% and elution flow rate isl. 5mL/min. China） on a variety of of extraction, Optimization, of ammonium
出处
《绿色科技》
2015年第12期277-278,280,共3页
Journal of Green Science and Technology
关键词
脂肪酶
柱层析
系统分离
lipase
column chromatography
systematic separation
