摘要
在35℃、含固率10%、接种量20%的条件下,对碳氮比(C/N)分别为8、12、15的3组污泥进行97 d的厌氧消化试验,分析消化过程中p H、NH_4^+-N、挥发性固体(VS)去除率及产气情况,并采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术分析微生物群落结构的变化。试验结果表明,8~13 d完成系统的启动,且消化过程中没有产生酸抑制现象。3组试验的单位VS产气率分别达到572、492和621 m L/g;VS降解效率分别达到57.1%、51.9%和51.0%;C/N比为8时,累积产气量最多;3组试验污泥DGGE图谱显示,碳氮比越低,细菌群落多样性越丰富,优势菌群群落密度也越大。因此,C/N为8时高含固率污泥厌氧消化效果更好。
The anaerobic digestion experiments of sludge with different C/N（8,12 and 15）were conducted at 35 ℃ with TS10% and inoculation amount 20% for 97 days. p H,NH4＋^-N and removal rate of VS and biogas production were analyzed through the whole experimental period,and changes in community structure were analyzed using PCR-DGGE technique.Result showed it took 8-13 days to start system successfully,and the acid suppression was not found during the anaerobic digestion. The potential capacity of gas production were 572 m L/g VS,492 m L/g VS and 621 m L/g VS,and the degradation efficiency were 57.1%,51.9% and 51.0%,respectively. The cumulative gas production reached the maximum when C/N was8. It was found that the more microbial diversity and dominant flora with the reducing of C/N through the DGGE profiles. So the anaerobic digestion efficiency was better when C/N was 8.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2015年第10期48-52,共5页
Environmental Science & Technology
基金
山东省自然科学基金资助项目(ZR2011BM014)
关键词
C/N
高含固率
厌氧消化
氨氮
产气量
C/N
high solid content
anaerobic digestion
ammonia nitrogen
biogas production
