摘要
目的研究中华补血草[Limonium sinense(Girard)Kuntz]中主要抗癌活性成分之一异鼠李素诱导人结直肠癌(HCT116)细胞自噬的机理。方法采用四唑盐比色法(MTT)检测异鼠李素对HCT116细胞的抑制作用,单丹尸磺酰胺(MDC)染色法检测自噬小泡的形成,蛋白印迹法(Western blot)检测自噬相关蛋白LC3的表达情况。结果异鼠李素明显抑制HCT116细胞的增殖,且呈剂量依赖性效应。异鼠李素处理24 h后,自噬小泡形成增多,LC3蛋白表达增强。3-甲基腺嘌呤(3-MA)预处理能够明显降低异鼠李素对肿瘤细胞的抑制作用。结论实验结果显示中华补血草中的异鼠李素可以诱导HCT116细胞发生自噬,导致细胞死亡。
AIM To investigate the mechanism for the isorhamnetin-induced autophagy of human colon carcinoma cell line HTC116,which was one of the anticancer constituents from Limonium sinense（ Girard） Kuntze.METHODS（ MTT） assay was performed on the inhibitory effect of isorhamnetin against HCT116 cells; the formation of autophagic vacuoles and the expression of autophagy-related protein LC3 were assessed by monodansylcadervarine（ MDC） staining and Western blot,respectively. RESULTS Isorhamnetin obviously exhibited the inhibition on HCT116 cells proliferation in a dose-dependent manner. After being treated with isorhamnetin for 24 hours,both the fluorescence intensity of MDC and the expression of protein LC3 were significantly enhanced. Furthermore,the inhibitory effect of isorhamnetin against HCT116 cells was weakened as these cells were pretreated with 3-methyladenine（ 3-MA）. CONCLUSION Isorhamnetin can induce the autophagic cell death in HCT116.
出处
《中成药》
CAS
CSCD
北大核心
2015年第12期2596-2599,共4页
Chinese Traditional Patent Medicine
基金
国家自然科学基金资助项目(81102817)
关键词
中华补血草
异鼠李素
HCT116细胞
细胞自噬
Limonium sinense（Girard） Kuntze
isorhamnetin
human colon carcinoma cell line（HCT116 cells）
autophagy
