摘要
为了探讨添加不同水平L-赖氨酸的精液稀释液对杜泊羊鲜精液态保存下品质的影响,试验选用健康的杜泊种公羊3只,采集精液,等量分装,分别加入到含不同水平（0、0.1、0.2、0.3、0.4和0.5g）L-赖氨酸的120mL稀释液中,在0、6、24、30、48、54、72和78h对精子活力、质膜完整率和顶体完整率进行检测。结果表明,添加0、0.1、0.2g L-赖氨酸组精子活力较其他添加水平组高,添加0.4和0.5g L-赖氨酸组精子活力下降速度快,添加0.5g L-赖氨酸组精子活力在30h时降为0,添加0.4g L-赖氨酸组精子活力在48h时降为0;添加0.1g L-赖氨酸组精子有效存活时间和生存指数最高,与其他组间差异显著（P〈0.05）;质膜完整率0.1g组最高,贮存0~54h间与对照组间差异不显著（P〉0.05）,72h后与对照组间出现显著性差异（P〈0.05）,0.4和0.5g组最差,与其他组间差异显著（P〈0.05）;顶体完整率0.1g组最高,与对照组间差异不显著（P〉0.05）,0.4和0.5g组最差,二者差异不显著（P〉0.05）,除贮存0和6h外,与其他组间差异极显著（P〈0.01）。结果提示,稀释液中添加高浓度的L-赖氨酸对精液品质有抑制作用,当L-赖氨酸添加水平≥0.2g,精子品质显著下降,添加0.1g L-赖氨酸有助于提高杜泊羊鲜精液态保存的品质。
In order to explore the effect of different L-lysine levels added in semen dilution on the fresh Dorper sheep quality stored at liquid state,3health Dorper ram were used to collect semen which were equal-packaged and added to solution containing different levels（0,0.1,0.2,0.3,0.4and 0.5g）L-lysine in 120 mL diluent and at 0,6,24,30,48,54,72 and 78h,the sperm motility,membrane integrity and acrosome integrity were detected.The results showed that the sperm motility in groups added 0,0.1and 0.2g L-lysine were higher than other groups,and that in 0.4and 0.5g L-lysine groups decreased faster than other groups;The sperm motility in 0.5g L-lysine group reduced to 0at 30 h,and it reduced to 0at 48 hin the group added 0.4g L-lysine;The effective survival time and survival index in the group added 0.1g L-lysine was higher than other groups（P〈0.05）;The membrane integrity of 0.1g group was the highest,that had no significant difference with control group from 0to 54h（P〉0.05）,while after 72 hthe two groups had significant differences（P〈0.05）.The membrane integrity in 0.4and 0.5g groups were the worst,and that was significant different with other groups（P〈0.05）;The acrosome integrity of0.1g group was the highest which had no significant difference with control group（P〉0.05）,0.4and 0.5g groups were the worst,the difference between them was not significant（P〉0.05）,while was extremely significant difference with other groups except for 0and 6h（P〈0.01）.The results suggested that dilution added a high concentration of L-lysine could inhibit sperm quality,when the count of L-lysine was more than 0.2g,sperm quality was significantly decreased,adding0.1g L-lysine could improve Dorper sheep fresh sperm quality stored at liquid state.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第12期3308-3314,共7页
China Animal Husbandry & Veterinary Medicine
基金
赤峰市农牧科学研究院自选项目(NKZZ201405)
