摘要
目的 评价褪黑素对异氟醚麻醉老龄大鼠术后海马细胞凋亡的影响.方法 清洁级健康雄性老龄SD大鼠60只,18月龄,体重550～610 g,采用随机数字表法分为5组（n=12）：对照组（C组）、2％异氟醚麻醉组（I组）、2％异氟醚麻醉＋褪黑素组（IM组）、手术组（S组）和手术＋褪黑素组（SM组）.C组不做处理,IM组吸入2％异氟醚4h,S组吸入2％异氟醚4h后行背部手术.IM组和SM组麻醉前连续7d每天腹腔注射10 mg/kg褪黑素,C组、I组和S组给予等容量生理盐水.各组在麻醉次日开始Morris水迷宫实验,测定大鼠逃避潜伏期、原平台探索时间及穿越平台次数.水迷宫实验结束当日处死大鼠取海马组织,采用Western blot法测定海马Bcl-2、Bax、caspase-3表达水平.结果 与C组比较,I组麻醉后第1-3天逃避潜伏期延长,S组术后第1-5天逃避潜伏期延长,原平台象限探索时间缩短,穿越平台次数减少,I组和S组海马caspase-3和Bax的表达上调,海马Bcl-2表达下调,Bcl-2/Bax比值降低（P＜0.05）;与I组比较,IM组麻醉后第1-3天逃避潜伏期缩短（P＜0.05）,海马caspase-3、Bcl-2、Bax表达水平及Bcl-2/Bax比值差异无统计学意义（P＞0.05）;与S组比较,SM组术后第3-5天逃避潜伏期缩短,原平台象限探索时间延长,穿越平台次数增加,海马caspase-3和Bax的表达下调,Bcl-2表达上调,Bcl-2/Bax比值升高（P＜0.05）.结论 褪黑素可抑制海马细胞凋亡,改善异氟醚麻醉老龄大鼠术后认知功能.
Objective To evaluate the effect of melatonin on postoperative apoptosis in hippocampal cells of aged rats undergoing surgery under isoflurane anesthesia.Methods Sixty healthy male Sprague-Dawley rats, aged 18 months, weighing 550-610 g, were randomized into 5 groups （n=12 each） using a random number table： control group （group C）, 2% isofiurane anesthesia group （group I）, 2% isoflurane ＋ melatonin group （group IM）, surgery group （group S）, and surgery ＋ melatonin group （group SM）.Group IM inhaled 2% isoflurane for 4 h.Group S inhaled 2% isoflurane for 4 h, and then back surgery was performed.In IM and SM groups, melatonin 10 mg/kg was injected intraperitoneally everyday for 7 consecutive days before anesthesia, while the equal volume of normal saline was given in C, I and S groups.The cognitive function was assessed using the Morris water maze test on 2nd after anesthesia.The escape latency, space exploration time spent at the original platform quadrant, and frequency of crossing the original platform were recorded.After the end of the Morris water maze test, the rats were sacrificed on that day, and the hippocampi were isolated for detection of the expression of Bcl-2, Bax and caspase-3 by Western blot.Bcl-2/Bax ratio was calculated.Results Compared with group C, the escape latency was significantly prolonged on days 1-3 after anesthesia in group I, the escape latency was significantly prolonged, the space exploration time spent at the original platform quadrant was shortened,and the frequency of crossing the original platform was decreased on days 1-3 after operation in group S,and the expression of caspase-3 and Bax was significantly up-regulated, the expression of Bcl-2 was downregulated, and Bcl-2/Bax ratio was decreased in I and S groups （P〈0.05）.Compared with group I, theescape latency was significantly shortened on days 1-3 after anesthesia （P 〈 0.05） , and no significant change was found in the expression of caspase-3, Bcl-2 and Bax, and Bcl-2/Bax ratio in group
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2015年第11期1339-1343,共5页
Chinese Journal of Anesthesiology
基金
国家自然科学基金（30972835）
关键词
褪黑激素
异氟醚
麻醉
手术后并发症
认知障碍
细胞凋亡
海马
Melatonin
Isoflurane
Anesthesia
Postoperative complications
Cognition disorders
Apoptosis
Hippocampus
