摘要
目的分析宁夏回族自治区（宁夏）手足口病病例中柯萨奇病毒A10型（CV-A10）分离株的基因特征。方法于2013年1月--2014年12月，利用手足口病实验室网络监测系统，采集宁夏各市、县到当地人民医院就诊的手足口病病例标本，共采集2470份，其中粪便标本450份，咽拭子2020份。使用人横纹肌肉瘤细胞进行病毒分离，获得的各种毒株用兼并引物进行VPl区基因扩增和序列测定。测序结果利用Blast进行病毒型别鉴定后，对鉴定为CV—A10的所有毒株进行同源性和系统进化分析。结果共收集到非EV—A71和非CV。A16的肠道病毒标本450份，分离到CV．A1036株，其中，2013年6株，2014年30株。36株毒株间的核昔酸和氨基酸同源性分别为90．6％～100．0％和90．2％--100．0％。与A、B、C、D各型别代表株的同源性比较发现，36株毒株与C型代表株的核苷酸和氨基酸同源性最高，分别为90．2％～98．9％和95．7％--99．7％。系统进化分析显示，36株宁夏CV．AIO分离株与C基因型代表株处于同一分支。结论CV—A10是引起宁夏地区手足口病的常见病原体，本次分离到的CV—A10均属于C基因型，具有较宽的同源性范围。
Objective To investigate the genetic characteristics of coxsackievirus A10（CV-A10） strains isolated from hand, foot and mouth disease （HFMD） cases in Ningxia province. Methods Based on the HFMD laboratory network surveillance system, 2 470 patients clinical specimens including 450 faeces and 2 020 throat swaps were collected from various regions people＇s hospital in Ningxia Hui Autonomous Region during January, 2013 to December, 2014. All specimens were isolated using rhabdomyosarcoma cells. VP1 regional gene of isolated strains was amplified by RT-PCR using degenerate primers and sequenced. Sequences were compared with the database of GenBank by the Blast algorithm to identify the enterovirus genotypes. All the CV-A10 strains were performed the homology and phylogenetic evolution analysis. Results 450 specimens identified as non-EV-A71, non-CV-A16 enterovirus were collected and 36 CV-AI0 strains were isolated, 6 strains were isolated in 2013 and 30 strains were isolated in 2014. The homology of nucleotides and amino acids among 36 CV-A10 strains were 90.6%- 100.0% , and 90.2%-100.0%, respectively. Compared 36 strains with genotype A, B, C, D representative strains, it has the highest homology with the genotype C, the nucleotide and amino acids homogeneity were 90.2%-98.9% and 95.7%-99.7%. The phylogenetic tree showed 36 strains and genotype C representative strains located in the same evolutionary branch. Conclusion CV-A10 was one of the most common pathogen of HFMD in Ningxia Hui Autonomous Region. All CV-A10 strains belonged to genotype C and contained wide homology range.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2015年第12期1056-1060,共5页
Chinese Journal of Preventive Medicine
基金
宁夏回族自治区科研支撑项目（2013ZYS119）
关键词
手足口病
生物学监测
柯萨奇病毒感染
基因型
Hand, foot and mouth disease
Biosurveillance
Coxsackievirus infections
Genotype
