摘要
目的研究2012年-2013年北京市西城区食源性单核细胞增生李斯特菌的同源性和耐药情况。方法按GB4789.30—2010方法分离鉴定菌株,按照标准化的Lm-PFGE方法对分离到的13株单核细胞增生李斯特菌,进行脉冲场凝胶电泳分型,药敏试验采用K-B法和E-test法。结果单核细胞增生李斯特菌总检出率为9.85%,耐药率为46.15%,主要耐药的抗生素有环丙沙星、红霉素、氯霉素、左氧氟沙星、美罗培南和头孢噻肟,并出现了多重耐药株。13株单核细胞增生李斯特菌通过PFGE分成了6个型别,以GX6A16.CN0004为主要带型(7株),占总菌株数的53.85%。结论本城区2012年-2013年部分食品不同程度地受到单核细胞增生李斯特菌的污染,GX6A16.CN0004基因型为优势菌,2013年出现新的基因型,单核细胞增生李斯特菌对多种抗生素敏感。加强对新型基因型的监测和临床使用抗生素的管理。
Objective To investigate the genotype homologous and of antibiotic status of food-borne Listeria monocytogenes in Xicheng district of Beijing during 2012-2013. Methods The isolates were separated and identified by standard methods of GB 4789. 30—2010,and according to international standardized Lm-PFGE protocol,13 isolated Lm strains were characterized by pulsed-field gel electrophoresis（ PFGE）. Susceptibility tests were performed with the Kirby-Bauer（ K-B） method and E-test method. Results The overall detection rate of Listeria monocytogens was 9. 85%,with the resistance rates of46. 15%. The main antibiotic resistances were ciprofloxacin,erythromycin,chloramphenicol,levofloxacin,meropenem and cefotaxime,and multi-resistant strains appeared. 13 Listeria monocytogens strains were isolated into 6 different genotypes by using PFGE,of which GX6A16. CN0004 is the main band type,accounting for 53. 85% in the total strains. Conclusion Foods in this district are contaminated by Listeria monocytogens in different degrees,GX6A16. CN0004 is the dominant genotype. A new genotype shows up in 2013. Listeria monocytogens in the district are susceptible to multi-antibiotics. And the monitoring of the new genotype and management of clinical use of antibiotics should be strengthened.
出处
《中国卫生检验杂志》
CAS
2015年第23期4143-4146,共4页
Chinese Journal of Health Laboratory Technology
关键词
单核细胞增生李斯特菌
脉冲场凝胶电泳法
耐药性
食源性致病菌
Listeria monocytogens
Pulsed-field gel electrophoresis
Antibiotic susceptibility
Food-borne pathogenic bacteria
