摘要
目的了解2010年-2014年北京市儿童感染性腹泻中病原菌构成和流行病学特征。方法收集北京市肠道门诊监测系统感染性腹泻患儿粪便标本,采用分离培养、生化鉴定和血清分型的方法对其进行霍乱弧菌、副溶血性弧菌、沙门菌、志贺菌和致泻性大肠埃希菌（DEC）等多种肠道致病菌的检测和分析。结果北京市2010年-2014年腹泻患儿粪便中共分离肠道致病菌679株,其中DEC位居所有检出菌的首位,占22.09%;其次是沙门菌、志贺菌,分别占19.88%和18.56%。腹泻患儿肠道致病菌感染人群主要集中在1岁~3岁幼儿,男女比例为1.12∶1。病原菌的检出具有明显季节性,夏、秋季为高峰。检出的DEC以肠致病性大肠埃希菌（EPEC）为主,占52.67%。沙门菌共分为34种血清型,以肠炎沙门菌为主,占32.59%。志贺菌以宋内志贺菌为主,占80.95%。结论北京市腹泻患儿病原菌感染以幼儿为主,男性多见,夏、秋季高发,病原菌谱以DEC为主。
Objective To understand pathogenic bacteria composition and epidemiological characteristics of infectious diarrhea caused by pathogenic bacteria among children in Beijing from 2010 to 2014. Methods The sporadic diarrheal patient＇ s rectal swabs and stool specimens were collected from enteroclinic surveillance system with infectious diarrhea among children. Enteric multiple pathogens including Vibrio cholerae, Vibrio parahaemolyticus, Salmonella, Shigella and diarrheagenic Escherichia coli were detected and analyzed from by the isolation culture, biochemical identification and serotyping. Results 679 strains were detected from infectious diarrhea among children in Beijing from 2010 to 2014. Diarrheagenic Escherichia coli（DEC） account- ing for 22.09% was the most frequently pathogen,followed by Salmonella（ 19.88% ） and Shigella（ 18.56% ）. Enteric patho- genic bacteria among children distributed mainly one to three years old children and the male and female ratio was 1.12 ： 1. The detection of pathogenic bacteria had obvious seasonal, summer and autumn was the peak. Enteropathogenic Escherichia coli （52.67%） was the main of diarrheagenic Escherichia coli, Salmonella Enteritidis （32.59%） was the main of 34 serotypes of Salmonella, Shigella Sonnei （80.95%） was the dominant serotype of Shigella. Conclusion The main age group of children infectious diarrhea were young children in Beijing, high incidence occured male children in summer and autumn. The dominant strain was diarrheagenic Escherichia coll.
出处
《中国卫生检验杂志》
CAS
2015年第22期3913-3917,共5页
Chinese Journal of Health Laboratory Technology
基金
"艾滋病和病毒性肝炎等重大传染病防治"国家科技重大专项(No.2012ZX10004215-003-001)
北京市高层次卫生技术人才培养计划(2013-3-009)
关键词
儿童
感染性腹泻
病原菌
监测
Children
Infectious diarrhea
Pathogenic bacteria
Surveillance
